Correlation Analysis between Vitamin B-group and Plasma Homocysteine in Non-smoking and Smoking Subjects

吸菸與非吸菸族群之血漿維生素B群與同半胱胺酸濃度之相關分析

陳暉雯(Haw-Wen Chen) ； 郭珍菱(Chen-Ling Kuo) ； 王佩云(Pei-Yun Wang) ； 劉青山(Chin-San Liu)

《The Changhua Journal of Medicine》 8卷3期 (2003/07) Pp. 141-148

摘要

背景及目的：吸菸是造成心血管疾病主要原因之一，而其中吸菸誘發動脈硬化之可能機轉是經由影響血漿同半胱胺酸代謝途徑，尤其與維生素B群濃度有關，本研究主要觀察健康族群中吸菸、同半胱胺酸濃度及維生素B群濃度之間的相互關係。
方法：176名健康者其中包括50名吸菸者和126名非吸菸者，此族群中已排除遺傳疾病，糖尿病、高血壓、高血脂、新陳代謝或心血管疾病，且無酗酒或維生素補充紀錄者。利用HPLC和RIA檢測血漿同半胱胺酸、維生素B6、維生素B12、與葉酸的濃度。
結果：研究顯示吸菸健康族群以男性為主並有較高之肉類攝取，經控制此兩干擾因子，發現吸菸族群血漿中維生素B6、維生素B12、與葉酸濃度皆顯著下降，但血漿同半胱胺酸濃度顯著增高，然而經由多因素迴歸分析，發現年齡和性別是非吸菸族群中決定血漿同半胱胺酸濃度之顯著獨立因子；然而在吸菸族群多因素迴歸分析中，卻發現葉酸是吸菸族群中唯一決定血漿同半胱胺酸濃度之顯著獨立因子。
結論：吸菸族群之維生素B群濃度皆顯著降低，其中葉酸缺乏可能與血漿同半胱胺酸濃度升高有關。

關鍵字

吸菸 ； 同半胱胺酸 ； 維生素B群

並列摘要

Background and purpose: Cigarette smoking is thought to be one of the major risk factors for cardiovascular disease. It has been related to the increase of plasma total homocysteine, which is thought to be affected by the plasma vitamin B-group. The aim of this study was to investigate the correlation between plasma B vitamins and homocysteine in smoking and non-smoking individuals.
Methods: A total of 176 subjects, consisting of 50 smokers and 126 non-smokers, were recruited. These subjects were free of genetic disorders, hyperlipidemia, diabetes, hypertension, and cardiovascular disease. Plasma homocysteine, vitamin 12 vitamin B6, and folic acid concentration were analyzed by HPLC and RIA.
Results: High meat consumption and gender were two confounding factors in smokers. After adjustment of the confounding factors, there was a significant increase in the plasma level of homocysteine and a decrease in the plasma concentration of vitamins B6, B12, and folic acid in smokers compared to non-smokers. Multiple linear regression analysis showed that age and gender were two independent risk factors for plasma homocysteine level in the whole and non-smoking groups. However, plasma folic acid was the only one independent risk factor for the plasma homocysteine level in the smoking group.
Conclusion: Levels of plasma vitamins B6, 12, and folic acid were significantly decreased in smokers compared to non-smokers. However, folic acid is the only significant independent risk factor for plasma homocysteine level in smokers.

並列關鍵字

cigarette smoking ； homocysteine ； vitamin B-group