以蚜蟲（半翅目：常蚜總科）及其初級內共生菌之分子特徵探討臺灣地區蚜蟲之系統發生

蚜蟲隸屬於半翅目（Hemiptera）胸喙亞目（Sternorrhyncha）常蚜總科（Aphidoidea）。目前臺灣有3科11亞科的蚜蟲，本研究取樣常蚜科（Aphididae）6亞科33種蚜蟲，外群為球蚜科（Adelgidae）鐵杉球蚜（＂Adelges tsugae＂），利用合併四段分子特徵分別為蚜蟲初級內共生菌（＂Buchnera aphidicola＂）之16S rDNA、蚜蟲粒線體COI（包含上游基因條碼及下游）及COII、蚜蟲核基因EF1-α，建構系統發生樹以釐清其高階親緣關係。最大簡約及貝氏系統發生分析顯示亞科級的分群與前人以形態或分子特徵之研究明顯不同，斑蚜亞科（Drepanosiphinae）位於系統樹之基部，而非與常蚜亞科（Aphidinae）位於末端的單系群內。常蚜亞科之分群結果為支持常蚜族（Aphidini）及其亞族（常蚜亞族（Aphidina）及縊管蚜亞族（Rhophlosiphina））之單系群關係，且網管蚜族（或稱長管蚜族、Macrosiphini）亦為單系群，與形態特徵結果一致。根據雞屎藤蚜（＂Aulacorthum nipponicum＂）與光額蚜屬（＂Acyrthosiphon＂）在系統樹上之相對關係，支持將雞屎藤蚜由粗額蚜屬（＂Aulacorthum＂）移至新粗額蚜屬（＂Neoaulacorthum＂）。

關鍵字

蚜蟲初級內共生菌；球蚜科；最大簡約；貝氏推論；基因條碼

並列摘要

The three families of aphids (Hemiptera: Sternorrhyncha: Aphidoidea) from Taiwan are divided into 11 subfamilies. This study presents the higher-level phylogenetic relationships among 6 subfamilies and 33 species, excluding the outgroup ＂Adelges tsugae＂ (Adelgidae), using a combined molecular dataset (16S ribosome DNA of ＂Buchnera aphidicola＂, DNA Barcoding cytochrome oxidase I and downstream, cytochrome oxidase II, elongation factor 1-α sequences). The maximum parsimony and Bayesian phylogenetic analysis revealed that the higher-level relationships among Aphididae were different from previous morphological or molecular classifications. Drepanosiphinae are on the basal clade, rather than in the monophyletic group with Aphidinae. Analyses supported the monophyly of Aphidini and its subtribes, Aphidina and Rhopalosiphina. Macrosiphini were also monophyletic. Our findings of the relative relationship of ＂Aulacorthum nipponicum＂ and ＂Acyrthosiphon＂ spp. on our phylogenetic trees, supports moving ＂Au. nipponicum＂ from ＂Aulacorthum＂ to the new genus ＂Neoaulacorthum＂.

並列關鍵字

＂Buchnera aphidicola＂； Adelgidae ； maximum parsimony ； Bayesian inference ； DNA barcoding

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以蚜蟲（半翅目：常蚜總科）及其初級內共生菌之分子特徵探討臺灣地區蚜蟲之系統發生

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