耐力性運動誘發的氧化壓力對人體免疫細胞DNA之影響

Effects of Oxidative Stress Induced by Endurance Exercise on DNA in Human Immune Cells

黃國欽(Kuo-Chin Huang) ； 邱亦涵(Yi-Han Chiu) ； 李永祥(Yong-Shing Lee) ； 徐廣明(Kuang-Ming Hsu) ； 徐台閣(Tai-Ger Hsu)

《北體學報》 11期 (2003/12) Pp. 41-51

https://doi.org/10.6167/TPEC/2003.11.5

摘要

Many studies demonstrated that oxidative stress was associated with strenuous exhaustive exercise due to increasing aerobic metabolism. This result may be attributed by oxidative DNA damage. However, little information is available on oxidative damage to leukocyte DNA induced by exhausted endurance exercise. The purpose of this study was to examine the effects of oxidative stress induced by Marathon exercise on DNA in human immune cells. The 42.195 km marathon race was used as a model for detection of this phenomenon.. Eight endurance runners were recruited from 2002 Taipei International Marathon Race to take part in this study. Venous blood samples were collected from each subject 12 hours before, immediately after running, 24 hr, and 120 hr Peroxide (H2O2), reduced glutathione (GSH) concentration and levels of oxidative DNA damage in leukocytes were measured by flow cytometery. After the race 24 hr, H2O2 concentration in human leukocytes was significantly increased. Concerning GSH concentration in leukocyte there was significant enhanced after race immediately, but GSH concentration quickly decreased following race 24 hr. The levels of oxidative DNA damage in leukocytes were considerably declined after race immediately, whereas significantly amplified after the race 24 hr and eventually recovered usual levels till 120 hr. In conclusion, oxidative stress induced by endurance exercise may be responsible for oxidative damage to DNA in human immune cells. This phenomenon is worthy to further study whether to be related with immune dysfunction after strenuous exercise.

關鍵字

耐力性運動 ； 氧化壓力 ； 免疫細胞 ； DNA損傷

並列摘要

Many studies demonstrated that oxidative stress was associated with strenuous exhaustive exercise due to increasing aerobic metabolism. This result may be attributed by oxidative DNA damage. However, little information is available on oxidative damage to leukocyte DNA induced by exhausted endurance exercise. The purpose of this study was to examine the effects of oxidative stress induced by Marathon exercise on DNA in human immune cells. The 42.195 km marathon race was used as a model for detection of this phenomenon.. Eight endurance runners were recruited from 2002 Taipei International Marathon Race to take part in this study. Venous blood samples were collected from each subject 12 hours before, immediately after running, 24 hr, and 120 hr Peroxide (H2O2), reduced glutathione (GSH) concentration and levels of oxidative DNA damage in leukocytes were measured by flow cytometery. After the race 24 hr, H2O2 concentration in human leukocytes was significantly increased. Concerning GSH concentration in leukocyte there was significant enhanced after race immediately, but GSH concentration quickly decreased following race 24 hr. The levels of oxidative DNA damage in leukocytes were considerably declined after race immediately, whereas significantly amplified after the race 24 hr and eventually recovered usual levels till 120 hr. In conclusion, oxidative stress induced by endurance exercise may be responsible for oxidative damage to DNA in human immune cells. This phenomenon is worthy to further study whether to be related with immune dysfunction after strenuous exercise.

並列關鍵字

endurance exercise ； oxidative stress ； immune cells ； DNA damage

參考文獻

呂鋒洲、徐台閣、徐廣明、鄭鴻文(2001)。穀胱甘肽對運動的影響。中華體育季刊。52，85-90。

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