簡訊：應用口蹄疫病毒3AB蛋白進行ELISA鑑別診斷豬隻自然感染及施打疫苗後之免疫反應

Short Communication: Differentiation of Foot-and-Mouth Disease Virus-infected from Vaccinated Pigs Using Non-structural Protein 3AB as Antigen in ELISA

楊莉嘉(Li-Chia Yang) ； 黃淑敏(Sue-Min Haung) ； 廖培志(Pei-Chih Liao) ； 黃金城(Chin-Cheng Huang) ； 鍾文彬(Wen-Bin Chung)

《臺灣獸醫學雜誌》 29卷2期 (2003/06) Pp. 168-173

https://doi.org/10.7009/TVJ.200306.0168

摘要

本試驗應用大腸桿菌表現口蹄疫（FMD）病毒非結構蛋白3AB作為抗原，發展出鑑別診斷自然感染及疫苗施打後之免疫反應試劑。首先，將O/TAW/97病毒株之3AB基因應用pET43.1a載體進行選殖，以大腸桿菌進行蛋白質表現，應用純化後的3AB蛋白作為抗原進行雙抗體三明治式酵素連結免疫吸附試驗（ELISA），並以標準陽性及陰性血清進行測試。結果發現，其可區分自然感染及疫苗免疫之動物，初步之敏感性及特異性分別為94.3%及97.9-100%。應用此試劑於台灣豬隻血清樣本之測試，結果發現，3AB抗體逐漸從豬群中消失，顯示病毒可能已從豬場清除。此亦顯示，本試劑可作為口蹄疫撲滅計劃中成效追蹤之用。

關鍵字

酵素連結免疫吸附試驗 ； 口蹄疫 ； 非結構蛋白 ； 豬隻

並列摘要

The E. coli expressed non-structural protein 3AB of foot-and-mouth disease (FMD) virus was used as the antigen in an enzyme-linked immunosorbent assay (ELISA) for the differentiation of virus-infected from vaccinated pigs. The 3AB gene of O/TAW/97 virus strain was cloned into pET43.1a plasmid, which was then transformed into E. coli for protein expression. The expressed and purified 3AB protein was used as the antigen in a modified double-antibody sandwich ELISA. This assay could differentiate vaccinated from convalescent pigs by testing a set of positive and negative reference sera. The preliminary sensitivity and specificity of the ELISA were 94.3% and 97.9-100%, respectively. The application of this diagnostic kit to field samples showed that the positive reactors gradually and steadily disappeared from the pig populations in Taiwan, indicating that virus might be gradually eliminated from the pig herds. The results demonstrated that this detection system could be a useful tool in monitoring the progress of the FMD eradication program.

並列關鍵字

ELISA ； Foot-and-mouth disease ； Non-structural protein ； Pig