The Antibiotic Susceptibility and Expression of Amoxicillin-Resistant Genes in Vibrio Strains Isolated from Hard Clams, Meretrix in Taiwan

本研究目的在於调查台灣地區文蛤養殖埸之弧菌分佈、抗藥情形及b/a(下标 SHV)和b/a(下标 TEM)在矍艘和染色艘的表现。205文蛤弧菌分離株中主要以Vibrio alginolyticus(103/205)爲主。利用連續平板稀釋法来偵测最小抑菌溫度，結果發现幾乎大部分的弧菌對xolinic acid具敏感性，而對amoxicillin則具有抗性，其中有77株對amoxicillin具有抗性。對amoxicillin產生抗性之主要原因爲β-lactamase的産物所致，然而藉由amoxicillin-clavul-anic acid試驗中發现有12株弧菌爲無法测得β-lactamase活性，利用PCR研究抗amoxicillin弧菌之抗amoxicillin基因b/a(下标 SHV)和b/a(下标 TEM)表现在，發現b/a(下标 SHV)位於質體和染色體，而b/a(下标 TEM)谨位於染色體。

關鍵字

抗菌劑； bla(下标 SHV) ； bla(下标 TEM) ；文蛤；弧菌；抵抗

並列摘要

The aims of this study were to assess the distribution of Vibrio spp. isolated from cultured clams in Taiwan, investigate its resistance to drugs, and determine the expression of the b/a(subscript TEM) and b/a(subscript SHV) genes in the plasmid and/or chromosome. An analysis of the 205 Vibrio strains that were isolated from clams showed that V. alginolyticus (103/205) was the dominant species. The minimal inhibitory concentration (MIC) of these antibiotics was determined using the serial plate dilution method and the results indicated that most Vibrios were sensitive to oxolinic acid and least sensitive to amoxicillin. Seventy-seven Vibrios strains were highly resistant to amoxicillin. The resistance of amoxicillin is mostly due to the production of β-lactam ase, however, there were 12 β-lactamase negative Vibrios stains resistant to amoxicillin and clavulanic acid. An investigation of the b/a(subscript TEM) and b/a(subscript SHV) genes coding for β-lactamase in amoxicillin-resistant strains revealed that the b/a(subscript SHY) gene was located in the plasmid and chromosome, but the b/a(subscript TEM) gene was only found in the chromosome.

並列關鍵字

antibiotics ； bla(subscript SHV) ； bla(subscript TEM) ； clam ； Vibrios ； resistance

國際替代計量

The Antibiotic Susceptibility and Expression of Amoxicillin-Resistant Genes in Vibrio Strains Isolated from Hard Clams, Meretrix in Taiwan

全文下載

主題瀏覽