離乳仔豬併欄時以Azaperone處理對血漿皮質醇與下視丘－腦下垂體－腎上腺軸緊迫相關基因表現變化之影響

Effect of Azaperone on Plasma Cortisol and Stress-related Gene Expression in the Hypothalamus-Pituitary-Adrenal Axis in Co-mingling Weaned Pigs

游建和(Chien-Ho Yu) ； 蕭世烜(Shih-Hsuan Hsiao) ； 陳靜宜(Ching-Yi Chen) ； 張志成(Chih-Cheng Chang)

《臺灣獸醫學雜誌》 34卷3期 (2008/09) Pp. 177-183

https://doi.org/10.7009/TVJ.200809.0177

摘要

This study was conducted to investigate the effect of azaperone on the plasma cortisol and stress-related gene expression in the hypothalamus-pituitary-adrenal (HPA) axis alteration in co-mingling weaned pigs. Eighteen 28 days old piglets from 6 litters were allocated to 3 groups, S: co-mingled group, T: azaperone treatment group and C: piglets stayed beside their dams in the far- rowing house. Blood samples were collected prior and 2 hours after co-mingling to determine the plasma cortisol change. After the second blood sampling, all animals were electrically euthanized and the HPA tissues were quickly removed for target gene mRNA quantification by real-time RT-PCR. The results showed that the concentrations of cortisol in S group are significantly higher (P＜0.05) than those in other groups. The gene expressions of hypothalamic c-fos in S and T group are down- regulated, and expressions of pituitary CRHR1 in S and T groups were up-regulated when compared to that in the C group. No significant differences on all these genes between S and T group were found. Thus, the co-mingling process was stressful to the weaned pigs, and azaperone may reduce the stress without influencing the stress-related gene expressions in the HPA axis.

關鍵字

併欄 ； 下視丘－腦下垂體－賢上腺軸 ； azaperone

並列摘要

This study was conducted to investigate the effect of azaperone on the plasma cortisol and stress-related gene expression in the hypothalamus-pituitary-adrenal (HPA) axis alteration in co-mingling weaned pigs. Eighteen 28 days old piglets from 6 litters were allocated to 3 groups, S: co-mingled group, T: azaperone treatment group and C: piglets stayed beside their dams in the far- rowing house. Blood samples were collected prior and 2 hours after co-mingling to determine the plasma cortisol change. After the second blood sampling, all animals were electrically euthanized and the HPA tissues were quickly removed for target gene mRNA quantification by real-time RT-PCR. The results showed that the concentrations of cortisol in S group are significantly higher (P＜0.05) than those in other groups. The gene expressions of hypothalamic c-fos in S and T group are down- regulated, and expressions of pituitary CRHR1 in S and T groups were up-regulated when compared to that in the C group. No significant differences on all these genes between S and T group were found. Thus, the co-mingling process was stressful to the weaned pigs, and azaperone may reduce the stress without influencing the stress-related gene expressions in the HPA axis.

並列關鍵字

co-mingling ； HPA axis ； azaperone