以阻斷型ELISA檢測抗家禽流行性感冒H5亞型抗體的臨床應用

Clinical Use of a Blocking ELISA for Detecting Anti-H5 Avian Influenza Antibody

朱文玉(Wen-Yu Chu) ； 陳奕成(Yi-Cheng Chen) ； 王金和(Ching-Ho Wang)

《臺灣獸醫學雜誌》 34卷4期 (2008/12) Pp. 248-254

https://doi.org/10.7009/TVJ.200812.0248

摘要

本研究之目的為評估全病毒阻斷型酵素聯結免疫吸附法(Virus-B-ELISA)之檢測效力，並簡化此Virus-B-ELISA以減短操作時間。方法為收集雞隻田間血清樣本，分別進行Virus-B-ELISA 及血球凝集抑制試驗(hemagglutination inhibition test, HI test)，並以HI test之結果作為金標準，計算出Virus-B-ELISA 相對於HI test之敏感性及特異性。本研究亦針對此Virus-B-ELISA之病毒塗鍍步驟加以簡化，且使用已知H5抗體陰性或陽性之血清，分別進行原始Virus-B-ELISA及簡化之Virus-B-ELISA檢測，以比較二者間之差異。結果發現原始Virus-B-ELISA檢測雞隻血清之敏感性與特異性若與HI test相較分別為95.76% (113/118)和91.47% (268/293)，步驟簡化前後Virus-B-ELISA之值為0.9539，顯示二種檢測之結果近吻合。以本實驗室製作之簡化阻斷型Virus-B-ELISA能更快速地偵測出H5亞型AIV抗體。

關鍵字

家禽流行性感冒病毒 ； 酵素聯結免疫吸附法 ； H5亞型單株抗體 ； 血球凝集素

並列摘要

The purposes of this study were to evaluate the efficiency of the blocking enzymelinked-immunosorbent assay by coating with the whole virus as the antigen (Virus-B-ELISA) and to modify the procedure to save time. The hemagglutination inhibition (HI) test was taken as the standard for calculating the sensitivity and specificity of the Virus-B-ELISA for detection of field chicken sera. This study simplified the virus coating step of the Virus-B-ELISA by drying and compared with that before drying. In original Virus-B-ELISA, the sensitivity and specificity based on the HI test was 95.76% (113/118) and 91.47% (268/293), respectively. The Kappa value between original Virus-B-ELISA and simplified Virus-B-ELISA was 0.9539, which reveals the latter assay provided high perfect consistency. Simplified Virus-B-ELISA provides way in the detection of AIV-H5 subtype antibody.

並列關鍵字

avian influenza virus ； enzyme-linked-immunosorbent assay ； H5-subtype monoclonal antibody ； hemagglutinin