- 期刊
口蹄疫O/Taiwan/97病毒、豬水疱病病毒感染豬隻之臨床病理研究
Clinical Features and the Activity of Serum Enzymes in the Pigs Infected with the Porcinophili Foot-and-Mouth Disease Virus and Swine Vesicular Disease Virus
摘要
19頭30至50公斤第二代SPF保育豬，分成兩組分別人工接種親豬性口蹄疫O/Taiwan/97病毒、豬水毒病病毒，於第0、1、2、3、4、5、6、8、12、15、19、26、35及50天採血，進行血清生化酵素值的測定，分析及比對標準參考值。結果發現包括肌酸活毒(creatine kinase, CK)、乳酸脫氫毒(lactate dehydrogenase, LDH)、天門冬酸鹽轉氨毒(aspartate aminotransferase, AST)與氨基丙酸轉氨毒(alanine aminotransferase, ALT)於病毒感染後在統計學上有明顯之變化。口蹄疫O/Taiwan/97病毒接種組豬隻於1Days post-infection (DPI)時ALT即上升，5 DPI達到高峰後逐漸下降；AST在3 DPI時迅速上升，4、5 DPI達到高峰；CK在4DPI迅速上升，5 DPI達到高峰；LDH則遲至5DPI才上升，6至15 DPI皆維持於高值。豬水毒病毒接種組豬隻之AST、ALT、LDH在感染後其活性微幅上升。可知口蹄疫O/Taiwan/97病會導致豬隻四種血清酵素活性上升，此可應用於病毒早期感染及區別豬口蹄疫與豬水毒病之快速診斷參考數據。
並列摘要
Nineteen 30 to 50 kg body weight secondary specific pathogen free (SPF) pigs were divided into 2 groups and were artificially infected with the porcinophilic O/Taiwan/97 strain of foot- and-mouth virus (FMDV) and swine vesicular disease virus (SVDV), respectively. Serum samples of the pigs were collected on day 0, 1, 2, 3, 4, 5, 6, 8, 12, 15, 19, 26, 30, 44, and 50 days post- infection (DPI) for serum enzyme activity examination. Results showed that four enzyme activities released from damaged tissues, including creatine kinase (CK), lactate dehydronase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were detected in sera. In the group of pigs infected with FMDV, ALT activity increased on 1 DPI, and reached the peak on 5 DPI; AST activity increased sharply on 3 DPI and reached the peak around 4 to 5 DPI; CK activity increased sharply on 4 DPI and reached the peak on 5 DPI; LDH activity increased on 5 DPI and remained at high level from 6 to 15 DPI. On the other hand, in the group of pigs infected with SVDV, activities of AST, ALT, and LDH were found slightly increasing on 2, 3, 5, and 8 DPI; the CK activity did not show any significant changes. It can be seen that the activities of these four enzymes will be released substantially from the damaged muscles into serum after infection with the OlTaiwan/97 strain of FMDV. Thus, we suggest that early and differential diagnosis of FMD and SVD can be accomplished by measuring the activities of CK, LDH, AST, and ALT.
延伸閱讀
- 張晏禎（2014）。亞臨床第二型豬環狀病毒感染豬的免疫狀態-血液病毒量對周邊血液單核細胞對致裂原及第二型豬環狀病毒刺激反應的影響〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2014.10487
- 李淑慧、鍾明華、黃天祥、林有良、杜文珍、曾惠鈴、林榮培、王之仰、林士鈺、朱瑞民（1998）。口蹄疫（臺灣分離病毒株）對豬致病機序之研究。中華民國獸醫學會雜誌，24()，6-6。https://doi.org/10.7009/JTCSVS.199812.0006
- 林正忠、劉哲宏、張照勤、李維誠、劉正義、陳德勲（2008）。不同來源之豬隻沙氏桿菌流行病學調查：探討台灣居民感染人畜共通沙氏桿菌症風險。台灣公共衛生雜誌，27(3)，243-249。https://doi.org/10.6288/TJPH2008-27-03-06
- Tsai, J. (2001). 消費者對動物傳染病爆發的反應毛豬口蹄疫爆發實證分析. 農業經濟半年刊, (69), 167-187. https://doi.org/10.7086/JAE.200106.0167
- Hieu, D. V. (2015). Expression of Genes Associated with Innate Immunity in Peripheral Blood Mononuclear Cells from Pigs Co-infected with Porcine Reproductive and Respiratory Syndrome Virusand Porcine Circovirus Type 2 [master's thesis, National Pingtung University of Science and Technology]. Airiti Library. https://doi.org/10.6346/NPUST.2015.00001