溶血性曼哈米亞桿菌（巴氏桿菌）Lpp38重組外膜蛋白之免疫原性分析

Genetic and Immunogenic Analysis of the Recombinant Lpp38 Outer Membrane Protein of Mannheimia (Pasteurella) Haemolytica

吳幸潔(Hsing-Chieh Wu) ； 林涵芸(Han-Yun Lin) ； 許世芳(Shih-Fang Shu) ； 劉世賢(Shyh-Shyan Liu) ； 莊秀琪(Hso-Chi Chaung) ； 朱純燕(Chun-Yen Chu)

《臺灣獸醫學雜誌》 35卷4期 (2009/12) Pp. 273-282

https://doi.org/10.7009/TVJ.200912.0273

摘要

Mannheimia (Pasteurella) haemolytica is a member of the Pasteurellaceae family, which is the major cause of acute fibrinous pneumonia in ruminants. The mortality of acute fibrinous pneumonia is high and vaccination is the most feasible strategy for controlling this disease. In this study, we isolated different strains of M.haemolytica from local cattle and sheep, cloned and expressed the 38kDa lipoprotein (Lpp38) to compare their amino acid sequences. Mice immunized with the recombinant Lpp38 (rLpp38) had a significantly (p＜0.01) higher titer of antigen-specific IgG2a than those immunized with M.haemolytica alone. Similar results were observed in a goat model post vaccination 2 weeks (p＜0.01). In summery, rLpp38 can be used as a potential immunogene to develop new vaccines for preventing infections induced by M.haemolytica.

關鍵字

溶血性曼哈米亞桿菌 ； 巴氏桿菌 ； 38kDa脂蛋白 ； 疫苗

並列摘要

Mannheimia (Pasteurella) haemolytica is a member of the Pasteurellaceae family, which is the major cause of acute fibrinous pneumonia in ruminants. The mortality of acute fibrinous pneumonia is high and vaccination is the most feasible strategy for controlling this disease. In this study, we isolated different strains of M.haemolytica from local cattle and sheep, cloned and expressed the 38kDa lipoprotein (Lpp38) to compare their amino acid sequences. Mice immunized with the recombinant Lpp38 (rLpp38) had a significantly (p＜0.01) higher titer of antigen-specific IgG2a than those immunized with M.haemolytica alone. Similar results were observed in a goat model post vaccination 2 weeks (p＜0.01). In summery, rLpp38 can be used as a potential immunogene to develop new vaccines for preventing infections induced by M.haemolytica.

並列關鍵字

Lpp38 ； mannheimia pasteurella haemolytica ； vaccine