The aims of the clinical study were to modify and simplify a detection method for the antioxidant, reduced glutathione (GSH), and to evaluate the levels of GSH in erythrocytes and aqueous humor in dogs with normal and cataractous lenses. One hundred and fifty eight dogs were included in this study. Erythrocytes from all 158 dogs were analyzed for GSH levels; 108 had normal lenses and 50 dogs had cataracts. Aqueous humor from thirty-two dogs was assayed for GSH levels; ten had normal lenses and 22 had cataracts. The average erythrocyte GSH level was 1,338.0±487.5 μM (mean±S.D.) in normal dogs and 927.4±504.5 μM in dogs with cataracts (p<0.05). The average aqueous GSH level in normal dogs was 90.3±70.9 μM and 92.5±80.0 μM in dogs with cataracts (p>0.05). This study served two purposes: to develop a sensitive assay for the detection of erythrocyte and aqueous humor GSH in dogs and to compare these values in dogs with and without cataracts. Dogs with cataracts demonstrated significantly lower erythrocyte GSH level than dogs without cataracts. Systemic GSH levels in dogs without cataracts may be one of the protective mechanisms against chronic oxidative stress and its resultant sequella such as cataractogenesis. However, aqueous humor GSH levels did not differ in dogs with or without cataracts. This may be explained by differences in endogenous levels of GSH or other antioxidants in the lens epithelial cells which may be different than that shown in aqueous humor.
The aims of the clinical study were to modify and simplify a detection method for the antioxidant, reduced glutathione (GSH), and to evaluate the levels of GSH in erythrocytes and aqueous humor in dogs with normal and cataractous lenses. One hundred and fifty eight dogs were included in this study. Erythrocytes from all 158 dogs were analyzed for GSH levels; 108 had normal lenses and 50 dogs had cataracts. Aqueous humor from thirty-two dogs was assayed for GSH levels; ten had normal lenses and 22 had cataracts. The average erythrocyte GSH level was 1,338.0±487.5 μM (mean±S.D.) in normal dogs and 927.4±504.5 μM in dogs with cataracts (p<0.05). The average aqueous GSH level in normal dogs was 90.3±70.9 μM and 92.5±80.0 μM in dogs with cataracts (p>0.05). This study served two purposes: to develop a sensitive assay for the detection of erythrocyte and aqueous humor GSH in dogs and to compare these values in dogs with and without cataracts. Dogs with cataracts demonstrated significantly lower erythrocyte GSH level than dogs without cataracts. Systemic GSH levels in dogs without cataracts may be one of the protective mechanisms against chronic oxidative stress and its resultant sequella such as cataractogenesis. However, aqueous humor GSH levels did not differ in dogs with or without cataracts. This may be explained by differences in endogenous levels of GSH or other antioxidants in the lens epithelial cells which may be different than that shown in aqueous humor.