Any rejection to collected specimens may prolong diagnosis and treatment time, as well as an increase in hospital spending and personnel loading. Any human factors during specimen collection should be monitored and reduced. From Jan. to April 2010, the refrigerated specimen rejection rate of the ward was 3.66%, higher then hospital average of 0.38%. Cause analysis attributed the issues to the following human errors: The recognition rate of nursing staffs on refrigerated specimen was merely 62.1%; the standard procedure of refrigerated specimen collection was improperly executed, and the specimen equipment was inappropriate. To counter these human errors, the following solutions were proposed: design specimen collection procedure, education training plans, DIY refrigeration specimen bag, and specimen collection guidelines. Upon implementation, the consequent rejection rate dropped form 3.66% to 0%, effectively improved the refrigerated specimen rejection rate and ensured the efficiency of medical treatment and the quality of services.