Allogeneic hand transplantation, a highly complex new surgical procedure, constitutes a major advancement in transplantation medicine. The preparation for the extraction of a donated hand is a critical indicator affecting patients' transplant surgery. This study revealed that the accuracy of surgical personnel's execution and their understanding of the extraction surgical procedure of the allogeneic hand was low. The causes included the following: 1) having limited knowledge of the storage method for a donated hand on ice, 2) lacking a container for storing and placing a donated hand, 3) and having limited knowledge of the repair surgery of a patient undergoing transplantation. The proposed improvement plan included conducting training and workshops on donated hand extraction, developing standard surgical procedures, purchasing containers for placing donated hands, and customizing prosthetic hands. After the improvement plan was implemented, the accuracy of the extraction procedure of surgical personnel increased from 50.3% to 99.6% and that of knowledge of extraction surgery increased to 99.1%. This indicates the value of this project in improving patient safety and medical quality associated with allogeneic donation extraction surgery.