電針梁丘穴對急性滑膜炎大鼠之下視丘影響

Effect of electroacupuncture stimulation of Liangqiu acupoint on the hypothalamus in rats with acute synovitis

鐘大勇(Dayong Zhong) ； 羅才貴(Caigui Luo) ； 周立偉(Li-Wei Chou)

《中華針灸醫學會雜誌》 21卷1-2期 (2018/03) Pp. 37-42

摘要

目的：探討對急性膝關節滑膜炎的大鼠，施予梁丘穴的電針刺激，觀察其影響大鼠下視丘β-內啡肽與腦啡肽的含量；亦藉由單穴與雙穴的刺激，探究電針治療急性炎症疼痛的機制。方法：將SD大鼠隨機分為正常組、模型組、誘發模型+單穴電針組、與誘發模型+雙穴電針組。採用酵素免疫分析法（ELISA）檢測大鼠下視丘β-內啡肽（β-EP）與腦啡肽（ENK）的含量。結果：與正常組比較，模型組大鼠下視丘β-內啡肽與腦啡肽的含量無顯著性差異(P ＞ 0.05)；誘發模型+單穴電針組下視丘β-內啡肽與腦啡肽含量比模型組顯著升高(P ＜ 0.01，0.01)；誘發模型+雙穴電針組下視丘β-內啡肽與腦啡肽含量比模型組顯著升高(P ＜ 0.05，0.01)；單穴電針組和雙穴電針組的下視丘β-內啡肽與腦啡肽含量無顯著性差異(P ＞ 0.05)。結論：單穴和雙穴電針梁丘均可緩解急性炎症疼痛，其機制可能是通過加強下視丘β-內啡肽與腦啡肽的合成和釋放。

關鍵字

膝關節急性滑膜炎 ； 電針 ； 腦啡肽 ； β-內啡肽 ； 梁丘

並列摘要

Objective: To investigate the electroacupuncture stimulation of Liangqiu acupoints in rats with acute knee synovitis, affecting the hypothalamus β-endorphin and enkephalin content . Through the stimulation of single and double acupoints, the mechanism of electroacupuncture treatment of acute inflammatory pain is explored. Methods: The SD rats were randomly divided into four groups: normal group, model group, model with single acupoint electroacupuncture group and model with double acupoints electroacupuncture group. Determination the content of β-endorphin (β-EP) and enkephalin (ENK) in the hypothalamus of rats by enzyme immunoassay (ELISA). Results: Compared with the normal group, there was no significant difference in the content of β-endorphin(β-EP) and enkephalin(ENK) in the hypothalamus of the model group (P ＞ 0.05). The content of β-endorphin and enkephalin in the hypothalamus of the model with single acupoint electroacupuncture group was significantly higher than that in the model group (P ＜ 0.01, 0.01). It also happened in double acupoints electroacupuncture group (P ＜ 0.05, 0.01). But there is no significantly difference between single and double acupoints electroacupuncture groups (P ＞ 0.05). Conclusions: Both single-acupoint and double-acupoints electroacupuncture stimulation of Liangqiu acupoint can alleviate acute inflammatory pain, and the mechanism may be through strengthening the synthesis and release of β-endorphin and enkephalin in the hypothalamus.

並列關鍵字

Acute knee synovitis ； Electroacupuncture ； Enkephalin ； β- endorphin ； Liangqiu

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