Ultrasound (US) has been well introduced in the treatment of musculoskeletal problems and sports area. As its development, the physician can investigate the joints, ligaments, tendons, peripheral nerves, and then perform the guided injections. The article reports the application of the ultrasound during the acupuncture and dry needling, which can be helpful for the differential diagnosis, anatomy identification, and dangerous prevention. The accuracy and safety can be much improved after the US application.