大眼華鳊親緣地理與族群遺傳結構之研究

Phylogeography and Population Genetic Structure of Sinibrama macrops Based on mtDNA

許桂菁(Kui-Ching Hsu) ； 蔡昆展(Kun-Chan Tsai) ； 邵廣昭(Kwang-Tsao Shao) ； 王建平(Jiang-Ping Wang) ； 林弘都(Hung-Du Lin)

《Bio Formosa》 40卷2期 (2005/12) Pp. 58-67

https://doi.org/10.6248/BF.2005.40(2).02

摘要

本研究利用粒線體DNA研究大眼華鳊之親緣地理與族群遺傳結構，Tajima's D統計上呈現顯著負值，高的平均單型多樣性(h=0.999)與低核苷酸歧異值(π=0.009)顯示大部分的族群歷經族群擴張。族群間分化指數平均值為0.583，顯示族群間具較低的基因交流而呈現高度分化。以聚類分析(neighbor-joining)法重建親緣關係圖，但並無法明顯再依地理區或河系分群，顯示大眼華鳊群正處於譜系排序(lineage sorting)的狀態。根據NCA法分析，顯示大眼華鳊族群曾經歷過去破碎化事件與長距離拓殖等歷史事件，在網狀親綠關係(network)的分析中發現淡水河與閩江的關係最為密切，顯示淡水河的大眼華鳊應由閩江擴散而來。

關鍵字

大眼華鳊 ； 親緣地理 ； 族群遺傳結構

並列摘要

The phylogeographical and population genetic structures of Sinibrama macrops were investigated based on genetic diversity of mtDNA. Significantly negative Tajima's D statistics based on mtDNA suggest that most populations originated from a small number of founders followed by demographic expansion. High haplotype diversity (h=0.999) and low nucleotide diversity (π=0.009) may be attributed to expansion after a period of low effective population size. MtDNA analyses revealing evident geographical structuring suggest limited gene flow between populations (F(subscript ST)=0.583). Neighbor-joining tree of mtDNA identified one lineage suggest all population revealing lineage sorting. Nested clade analyses revealing significant associations between genetic structure and geographical division identify past fragmentation and/or Long-distance colonization as major phylogeographical events that shaped the geographical distribution of this species. According the nested clade analyses, the populations of the DanShui River and the Mm River had the closed relationship. This result suggested the populations of the DanShui River would be originated from the Min River.

並列關鍵字

Sinibrama macrops ； phylogeography ； population genetic structure