- 期刊
Clinical Significance of Tumor Cell-lined Vessel in Colorectal Cancer: Correlation with Tissue-Infiltrated Macrophage and Hypoxia Inducible Factor-1alpha Expression
腫瘤細胞形成血管在大腸直腸癌的臨床意義與巨噬細胞及缺氧因子的關係
摘要
目的 最近研究指出某些腫瘤細胞形成血管與癌症進展有關。本實驗主要目的在於研究腫瘤細胞形成血管在大腸直腸癌的臨床意義與巨噬細胞及缺氧因子的關係。 方法 共58位大腸直腸癌的病理手術後標本，我們用免疫化學染色CD34和pan-CK來染腫瘤細胞形成血管，細胞缺氧用缺氧因子HIF-1α來表現，免疫化學染色CD68來染巨噬細胞，然則用統計學的方法來分析彼此的關係。 結果 腫瘤細胞形成血管在29.3%的腫瘤標本中可被發現。腫瘤細胞形成血管與腫瘤T stage有關(P=0.001)。在癌症組織中，巨噬細胞與腫瘤T stage (P=0.016)，淋巴腺轉移(P=0.034)，及遠端轉移(P=0.002)等有關。另外腫瘤細胞形成血管與缺氧因子之間亦有相關性(P=0.004)。 結論 本實驗發現癌症細胞內部的細微環境本身可以改變讓癌細胞更具侵略性。而對於大腸直腸癌的預後來說，腫瘤細胞形成血管、巨噬細胞及缺氧因子可以是有用的指標。
並列摘要
Purpose. Recent studies had suggested tumor cell-lined vessel may involve in cancer progression. Our aim was to study the role of tumor cell-lined vessel (TCLV) in colorectal cancer (CRC) and the association of TCLV with tissue-infiltrated macrophage (TIM) and hypoxia inducible factor-1 alpha (HIF-1α) expression. Methods. Immunohistochemical staining in 58 patients with CRC were evaluated. Double-stain of CD34 and pan-cytokeratin was used to identify TCLV which are lack of endothelium lining. Hypoxia was evaluated by examining HIF-1α expression in tumor cells. Monoclonal antibodies of CD68 were used to investigate the TIM. Their correlations with clinicopathologic features were analyzed with chi-square test. Results. TCLVs were identified in 17/58 (29.3%) of tumor samples. Presence of TCLV correlated significantly with T stage (P=0.001) and histologic differentiation (P=0.038). In cancer tissues, the mean TIM (cells/mm^2) was 143±49. There was significant correlation between TIM and T stage (P=0.016), lymph node state (P=0.034), and tumor metastasis (P=0.002). Significant association between HIF-1α expression and presence of TCVL was also noted (P=0.004). Conclusion. This study showed that cancer microenvironment could change tumor cells to a more aggressive behavior. The presence of TCVL, TIM, and HIF-1α expression are potentially useful markers of tumor progression in CRC.
延伸閱讀
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