超臨界二氧化碳萃取洛神花多酚成分及其抗氧化活性之研究

Study on the Relationships between Polyphenols Components and Antioxidant Activities in Hibiscus Sabdariffa Linnaeu Supercritical Carbondioxide Fluid Extrcts

高而仕

《美容科技學刊》 8卷3期 (2011/09) Pp. 106-127

https://doi.org/10.30001/JIES.201109.0009

摘要

二氧化碳超臨界萃取技術具有較低溫度，高擴散能力之特性，可將天然物中的熱不穩定性物質萃出，並保留活性成分，所以本研究以二氧化碳超臨界流體萃取技術萃取洛神花多酚類物質咖啡酸。洛神花所含有的多酚類物質具有許多的生物活性，其中的有效成分咖啡酸，具備有抗氧化、捕捉自由基、抑制酪氨酸酶的功能。將不同比例的水、乙醇作為共溶劑添加至二氧化碳超臨界流體中進行修飾，並且改變不同的臨界壓力、臨界溫度、與超臨界流體的流速藉此評估使用共溶劑修飾的超臨界流體萃取洛神花咖啡酸效率的最佳化條件。根據高效能液相層析儀針對萃取物的咖啡酸含量分析結果，可以得到的最佳化萃取條件為臨界壓力250bar、臨界溫度40℃、共溶劑乙醇濃度85%、共溶劑流量1.2ml/min。在抗氧化活性當中，其氧化還原能力、清除DPPH自由基及酪氨酸酶之能力測定發現當咖啡酸萃取率上升，清除DPPH自由基則提升，而氧化還原能力上咖啡酸萃取率也頗佳，但酪氨酸酶能力上，則看不出相同趨勢，但在HPLC圖譜上較複雜，可能萃取出含有較優異抑制酪氨酸酶之成分。

關鍵字

超臨界二氧化碳萃取 ； 洛神花 ； 多酚 ； 咖啡酸 ； 抗氧化

並列摘要

Supercritical carbon dioxide (SC-CO2) can provide a benefit of lower temperature and high diffusion ability in nature product extraction process, not only preserved the thermo unstable but also keep the bioactivity of the extracts. Owing to this, we use supercritical carbon dioxide extract the caffeic acid (CA), a kind of polyphenol, from Roselle. Caffeic acid has abilities with antioxidant, radical catching and tyrosinase inhibition. We add water and alcohol as the co-solvent to modify the polarity of the supercritical carbon dioxide, and use this modified supercritical carbon dioxide in the caffeic acid extraction with differ critical temperatures, critical pressures and flow rates. In the results of the further analysis by high performance liquid chromatography, the best optimization in modified supercritical carbon dioxide extraction of caffeic acid needs critical pressure at 250bar, critical temperature at 40℃, Co-solvent 85% ethanol as co-solvent at the flow rate 1.2ml/min. Among the antioxidant activity of its redox ability to remove DPPH Radicals and the ability to determine tyrosinase found that caffeic acid extraction rate, clear the DPPH Free radicals are improving, and redox ability of caffeic acid on the extraction rate is quite good, but tyrosinase Ability, then do not see the same trend, but more complicated on the HPLC chromatogram, may be extracted With excellent inhibition of tyrosinase than the ingredients.

並列關鍵字

supercritical carbon dioxide extraction method ； Hibiscus sabdariffa Linnaeus ； polyphenol ； caffeic acid ； antioxidant

參考文獻

連培榮、孫傳家(2009)。超臨界萃取的應用。科學發展。441，30-34。

Chen, C. C.,Hsu J. D.,Wang, S. F.,Chiang, H. C.,Yang, M. Y.,Kao, E. S.,Ho, Y. C.,Wang, C. J .(2003).Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits.Journal of agricultural and food chemistry.51,5472-5477.

El-Saadany, S. S.,Sitohy, M. Z.,Labib, S. M.,El-Massry, R. A.(1991).Biochemical dynamics and hypocholesterolemic action of Hibiscus sabdariffa (Karkade).Nahrung.35,567-576.

Hirunpanich, V.,Utaipat, A.,Morales, N. P.,Bunyapraphatsara, N.,Sato, H.,Herunsale, A.,Suthisisang, C.(2006).Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats.J Ethnopharmacol.103,252-260.

Haji Faraji, M.,Haji Tarkhani, A.(1999).The effect of sour tea (Hibiscus sabdariffa) on essential hypertension.J Ethnopharmacol.65,231-236.