Differentiation is a significant element in firm's development. I t can also assist the businesses to gain competitive advantage and generate the precise strategy. Thus in this paper, we propose the matrix model of differentiation in value chain to analyze the differentiation activities of convenience chain stores in Taiwan. The findings based on the interview of key person in convenience chain stores are that time, customers' demand and financial aspect impact on differentiation in value chain. For future research, we can apply quantitative research for testing results or expand participants in qualitative research.