高溫處理下小麥幼苗根部之抗氧化分析

Antioxidative Analysis of Root under High Temperature Treatment in Wheat Seedling

楊承運(Cheng-Yun Yang) ； 許奕婷(Yi-Ting Hsu)

《作物、環境與生物資訊》 16卷1期 (2019/03) Pp. 36-45

https://doi.org/10.30061/CEB.201903_16(1).0004

摘要

本研究之目的在探討高溫逆境對6種不同品系普通小麥（Triticum aestivum L.）幼苗根部生理性狀之影響。試驗中以水耕培養8天大幼苗為材料，比較32℃高溫處理和25℃常溫處理後，小麥各品系根部生長、蛋白質含量、氧化逆境與抗氧化系統等變化，其中氧化逆境之發生以過氧化氫與Malondialdehyde（MDA）為指標，抗氧化系統則分析抗氧化物Ascorbate（ASC）與Glutathione（GSH）含量，抗氧化酵素則偵測Superoxide dismutase（SOD）、Catalase（CAT）、Ascorbate peroxidase（APX）與Glutathione reductase（GR）的比活性。結果顯示高溫逆境會顯著抑制小麥幼苗的主根長度、側根數目與根系的乾、鮮重，但會增加過氧化氫與MDA含量。然而，即使是不同品系小麥，在高溫下根部蛋白質含量均明顯高於常溫處理，同時高溫也提高小麥幼苗根部抗氧化物ASC與GSH含量，以及抗氧化酵素GR、CAT與APX比活性。綜合上述，高溫可能藉由增加不同品系小麥幼苗根部的氧化逆境，進而抑制根部生長。

關鍵字

小麥幼苗 ； 根 ； 高溫逆境 ； 氧化逆境

並列摘要

This study aimed to compare the physiological and biochemical changes from six accessions of wheat (Triticum aestivum L.) seedling in roots under normal (25°C) or high temperature stress (32°C) condition. Hydroponically cultivated 8-day-old wheat seedlings were used to examine the dynamics of H_2O_2, Malondialdehyde (MDA), root growth, protein contents, antioxidant contents, and antioxidant enzyme activity. The results showed that high temperature increased the levels of H_2O_2 and MDA, two indicators of oxidative stress. High temperature stress also increased total protein content, levels of ascorbate and glutathione, as well as activity of antioxidant enzymes including superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase. Taken together, the result suggested that heat stress significantly induced oxidative stress in roots, consequently inhibited the growth and development of root tissues in wheat.

並列關鍵字

Wheat seedlings ； Root ； Heat stress ； Oxidative stress