以中部某區域醫院收集的非傷寒性沙門氏菌進行藥物敏感性及流行病學探討

Exploring the Drug Sensitivity and Epidemiological Characteristics of Nontyphoidal Salmonella Collected from a Regional Hospital in Central Taiwan

吳敏華(Min-Hua Wu) ； 林青毅(Ching-Yi Lin) ； 廖雅玲(Ya-Ling Liao) ； 余仁惠(Jen-Hui Yu)

《澄清醫護管理雜誌》 10卷1期 (2014/01) Pp. 10-14

摘要

BackgroundSalmonella is not only a very commonly observed zoonotic pathogen that exists extensively in the environment, but it is also a critical public health concern worldwide. The purpose of this study was to collect samples of nontyphoidal Salmonella in order to determine drug sensitivity and epidemiological features.Materials and methodsEpidemiologic data were obtained from 228 strains of nontyphoidal Salmonella collected between February 2008 and December 2009 in a regional hospital in central Taiwan. The agar disk diffusion method was used to determine drug sensitivity in these 228 strains of bacteria. In addition, Salmonella serogroups were identified using slide agglutination tests on the anti-serum of Salmonella groups. Salmonella DNA mapping was confirmed by pulsed field gel electrophoresis as recommended by the Center for Disease Control and Prevention. Results from the pulsed field gel electrophoresis were analyzed by BioNumerics software to verify the serotypes of Salmonella.ResultsAnalysis of the profiles of patients with the 228 strains of nontyphoidal Salmonella showed that most of the nontyphoidal Salmonella positive patients were between 0 and 15 years of age (N=140, 61.44%), and the second largest age group was that of patients older than 60 (N=42, 18.4%). In terms of gender, 124 of the patients were male (54%), the remaining 104 patients were female (46%). The two major sources of samples were stools (N=179, 78.5%) and blood (N=38, 16.7%). A total of 23 serotypes were found from the 228 strains of nontyphoidal Salmonella; the three most prevalent types were S. Enteritidis (38.2%, 87 out of 228), followed by S. Typhimurium (17.1%, 39 out of 228) and S. Newport (7.0%, 16 out of 228).

關鍵字

非傷寒性沙門氏菌 ； 血清群 ； 血清型

並列摘要

BackgroundSalmonella is not only a very commonly observed zoonotic pathogen that exists extensively in the environment, but it is also a critical public health concern worldwide. The purpose of this study was to collect samples of nontyphoidal Salmonella in order to determine drug sensitivity and epidemiological features.Materials and methodsEpidemiologic data were obtained from 228 strains of nontyphoidal Salmonella collected between February 2008 and December 2009 in a regional hospital in central Taiwan. The agar disk diffusion method was used to determine drug sensitivity in these 228 strains of bacteria. In addition, Salmonella serogroups were identified using slide agglutination tests on the anti-serum of Salmonella groups. Salmonella DNA mapping was confirmed by pulsed field gel electrophoresis as recommended by the Center for Disease Control and Prevention. Results from the pulsed field gel electrophoresis were analyzed by BioNumerics software to verify the serotypes of Salmonella.ResultsAnalysis of the profiles of patients with the 228 strains of nontyphoidal Salmonella showed that most of the nontyphoidal Salmonella positive patients were between 0 and 15 years of age (N=140, 61.44%), and the second largest age group was that of patients older than 60 (N=42, 18.4%). In terms of gender, 124 of the patients were male (54%), the remaining 104 patients were female (46%). The two major sources of samples were stools (N=179, 78.5%) and blood (N=38, 16.7%). A total of 23 serotypes were found from the 228 strains of nontyphoidal Salmonella; the three most prevalent types were S. Enteritidis (38.2%, 87 out of 228), followed by S. Typhimurium (17.1%, 39 out of 228) and S. Newport (7.0%, 16 out of 228).

並列關鍵字

nontyphoidal Salmonella ； serogroups ； serotypes