The purpose of this study was to 2016 annual the participation motivation and Well-being of the Taipei Expressway Marathon. In this study, In 2016 the Taipei Expressway Marathon participants for the study, for sample using the Internet sampling survey methods, Researcher send out 500 questionnaires, here were 380 valid questionnaires and valid response rate of 76 %. Statistics analysis method included descriptive statistics, factor analysis, item analysis, correlation analysis, reliability analysis, validity analysis, partial least squares and other statistics method were analyzed the questionnaires, after statistics analysis of the resulting data, the study found: The Taipei Expressway Marathon participants’ participated motivations for well-being were highly influential.