新型低溫顯微鏡之研製與性能測試

Development and Performance Test of a Novel Cryomicroscope

李柏廷(Po-Ting Lee) ； 楊江益(Chiang-Yi Yang)

《宜蘭大學生物資源學刊》 5卷1期 (2009/06) Pp. 15-22

https://doi.org/10.6175/job.2009.51.12

摘要

低溫顯微鏡系統是藉由軟體整合一個能控制溫度恆定以及升溫、降溫速率的冷凍台座，結合顯微鏡及影像輸出設備所構成的系統。傳統的低溫顯微鏡以液態氮進行系統的升溫及降溫控制。然而，高價位以及大體積使得傳統的低溫顯微鏡無法被廣泛地使用。本研究以控制熱電致冷晶片的表面溫度取代傳統的液態氮流體控制，研製的系統具有輕巧、安裝及操作維護容易、溫度控制精準及價格便宜等優點。目前研發的系統，可以準確地在每秒等速下降0.5℃/sec至3℃/sec的範圍內進行等速率降溫控制；而恒溫控制的結果，其與設定溫度的誤差皆控制在0.5℃以內。經以豐年蝦卵進行系統整合測試，結果發現應用本系統進行細胞內凍結特性之研究為可行。

關鍵字

低溫顯微鏡 ； 熱電致冷晶片

並列摘要

A cryomicroscope system was composed of a cold stage, a temperature-controlled (TEC) circuit, an image processing unit, and a software for system integration. The cooling and heating rates of a conventional cryomicroscope for the cryopreservation of biological cells are controlled by using liquid nitrogen. However, the conventional cryomicroscope can not be widely used by some laboratories for the reasons of high price and large size. The purposes of this study are to develop a cyromicroscope system based on thermoelectric cooling chip (TEC) instead of liquid nitrogen. TEC cryomicroscope has the advantage of small size, easy setup, precise temperature controlling, and low price. The developed system can achieve accurate control of cooling rate between 0.5°C/sec and 3°C/sec and isothermal control with root mean square errors lower than 0.5°C compared with the setting temperature. The results show that TEC cryomicroscope is viable for studying on intracellular ice formation phenomenon in biological cells, for example, Artemia shrimp in this study.

並列關鍵字

Cryomicroscope ； Thermoelectric cooler