In Vitro Plant Let Regeneration of the Medicinal Angelica Acutiloba K. Via Stem Explants

日本當歸（Angelica acutiloba K.）莖培植體之植株再生

黃馨慧(Shin-Hui Huang) ； 羅國卿(Kuo-Chin Lo) ； 陳泓墾(Hung-Ken Chen) ； 高建元(Chien-Yuan Kao)

《宜蘭大學生物資源學刊》 5卷1期 (2009/06) Pp. 41-45

https://doi.org/10.6175/job.2009.51.15

摘要

日本當歸莖段培植體，在0.6 mg/L 2,4-D（2,4-dichlorophenoxy acetic acid）、0.05 g/Ll抗壞血酸（ascorbic acid）和1.05 g/L檸檬酸（citric acid）的MS（Murashige and Skoog）培養基中，2週內可誘導出黃白色、緊密狀的癒合組織。癒合組織在原培養基中繼代培養，每月可增加2到4倍鮮重。將黑暗中生長的癒合組織移到光環境下，會生成綠色、結節狀的癒合組織並進一步自發性的分化形成多芽體。栘植多芽體培植體到含0.02 mg/L NAA（naphthyleneacetic acid）和0.1 mg/L kinetin的MS根誘導培養基時，可在3週內在莖的基部長出不定根而成為完整的植株。這些在試管內再生成功的完整植株，可以移植到盆土中作溫室栽培。

關鍵字

莖培植體 ； 癒傷組織 ； 藥用植物 ； 日本當歸 ； 植株再生

並列摘要

Callogensis, shoot proliferation and plant let regeneration have been achieved for Angelica acutiloba K. through stem explants. Various explants excised from leaf, stem and root were collected from established aseptic plants. The stem explants of Angelica acutiloba K. induced compact and yellow-white calli from the proximal ends on Murashige and Skoog (MS) salts supplemented with 0.6 mg/L 2, 4-D, 0.05g/L ascorbic acid and 1.05 g/L citric acid within two weeks in darkness. Calli were maintained by subculturing on the same medium for callus induction and proliferated 2-4 folds (fresh weight) in 1 month. Upon lighting condition, green compact calli were produced and turned into nodular appearance which subsequently differentiated into multiple shoots vigorously, typical of light-dependent development. Shoots with well-developed leaves were planted individually on a root-induction MS medium containing 0.02 mg/L naphthyleneacetic acid and 0.1 mg/L kinetin. Adventitious roots protruded from the basal parts of the stems within 3 weeks after transferring to the rooting medium. The in vitro regenerated plantlets were successfully transferred to soil.

並列關鍵字

stem explants ； callus ； medicinal plant ； Angelica acutiloba K. ； plant regeneration