酸鹼、熱處理及儲藏條件對微膠囊化牛初乳與乳清中IgG安定性之影響

Effects of pH, Thermal Processing, and Storage Conditions on the Stability of Immunoglobulin G (IgG) in Microencapsulated Bovine Colostrums and Whey

陳志瑋(Chih-Wei Chen) ； 沈子偉(Tzu-Wei Shen) ； 江淑華(Shu-Hua Chiang) ； 王秀育(Shiu-Yu Wang) ； 張基郁(Chi-Yue Chang)

《科學與工程技術期刊》 6卷4期 (2010/12) Pp. 59-66

https://doi.org/10.7117/JSET.201012.0059

摘要

本研究以取自母牛分娩後第2、3、4天分泌且等體積混合的乳汁作爲材料，以阿拉伯膠、β-環狀糊精或幾丁聚醣之10%水溶液，分別依1:4體積比和牛初乳或乳清混合，以冷凍及噴霧乾燥法進行微膠囊化，並探討pH值、熱處理及儲藏時間對微膠囊化初乳與乳清中IgG安定性之影響。結果顯示，在酸鹼安定性方面，未微膠囊化之初乳在pH 7~8有較高之IgG活性，經微膠囊化者則以阿拉伯膠所得者在pH 7~8時有較高之IgG活性。在熱安定性方面，阿拉伯膠和β-環狀糊精對於初乳或乳清之IgG均具有保護作用。在儲藏條件方面，儲藏溫度與包裝材質對於初乳或乳清之IgG殘存活性有顯著影響，在4℃儲存60天後之IgG活性較室溫高，以鋁袋包裝儲存者高於以透明塑膠袋包裝者，以阿拉伯膠微膠囊化之初乳在4℃儲存60天後之IgG殘存活性顯著高於未微膠囊化者。

關鍵字

牛初乳 ； β-環狀糊精 ； 幾丁聚醣 ； 阿拉伯膠 ； 微膠囊化

並列摘要

The bovine colostrums collected from the second, third, and fourth days postpartum were mixed with an equal volume and used as the raw materials. A 10% aqueous solution of gum arabic, β-cyclodextrin or chitosan was added in an equal volume of colostrums or whey, and microencapsulated through freeze-drying and spray-drying methods. The effects of pH, thermal processing, and storage conditions on the stability of the immunoglobulin G (IgG) in microencapsulated colostrums and whey were investigated. Results showed that non-microencapsulated colostrums were found to have higher IgG activity at pH 7~8 than at other pH values. The microencapsulated colostrums with gum arabic were found to have higher IgG residual activity at a pH 7~8. For thermal stability, gum arabic and β-cyclodextrin (β-CD) were found to have a protective effect on IgG activity of colostrums and whey. For storage conditions, storage temperature and packaging materials were found to have a significant impact on residual IgG activity of colostrums and whey. After being stored for 60 days at 4 ℃, the IgG activity was found to be higher than those stored at room temperature were. As for packaging materials, aluminum bag-packed colostrums and whey were found to have higher IgG activity than transparent plastic bag-packed colostrums. After storage for 60 days at 4 ℃, the microencapsulated colostrums with gum arabic were found to have significantly higher IgG residual activity than the non-microencapsulated colostrums.

並列關鍵字

bovine colostrums ； β-cyclodextrin β-CD ； chitosan ； gum arabic ； microencapsulation