Proteomic Analysis of PEG-simulated Drought stress-responsive Proteins of Rice Leaves Using a Pyramiding Rice Line at the Seedling Stage

水稻導入系幼苗葉片在PEG模擬乾旱脅迫條件下的蛋白組學分析

熊建華(Jian-Hua Xiong) ； 傅彬英(Bin-Ying Fu) ； 徐化學(Hua-Xue Xu) ； 李陽生(Yang-Sheng Li)

《Botanical Studies》 51卷2期 (2010/04) Pp. 137-145

摘要

乾旱是制約水稻產量的一個關鍵引物。為了調查水稻對乾旱脅迫的反應，我們以抗旱QTL導入系PD86為材料通過PEG 模擬乾旱脅迫，運用雙向電泳技術研究其蛋白水準上的表達變化。經過8 天的脅迫處理，我們發現有28個蛋白的表達發生了變化，其中23個蛋白表現上調，5個下調。通過質譜鑒定出其中的12個蛋白，這些蛋白參與抗氧化代謝，光合成，細胞骨架，防禦，蛋白代謝和信號傳導等功能路徑。鑒定的蛋白中，peroxiredoxin，ribonuclease 和 putative chitinase 這3個蛋白基因與導入系導入的親本抗旱QTL重疊，另外，voltage-dependent anion-selective channel protein, ribonuclease和putativechitinase 與已知的主效抗旱QTL位元點重合，顯示這些蛋白基因在抗旱機制中發揮重要作用。結合轉錄組水準的螢光定量分析的結果揭示了抗旱途徑在可能存在抗氧化代謝，光合成，蛋白降解，細胞骨架組織和細胞凋亡等機制。

關鍵字

乾旱 ； 抗旱 ； 雙向電泳 ； 蛋白組 ； 水稻 ； 質譜

並列摘要

Drought is one of the most severe limitations on the productivity of rice. To investigate the response of rice to drought stress, changes in protein expression were analyzed using a proteomic approach in a drought tolerant quantitative trait locus (DT QTL) pyramiding rice line PD86 by way of PEG simulated drought stress. After drought stress for 8 days, 23 proteins increased in abundance and the level of five proteins decreased. Twelve of the drought-responsive proteins were identified by mass spectrometry. These proteins are involved in redox metabolism, photosynthesis, cytoskeleton stability, defense, protein metabolism, and signal transduction. Among the identified proteins, peroxiredoxin, ribonuclease and putative chitinase, coincided with three QTL regions carried by PD86; in addition, voltage-dependent anion-selective channel protein, ribonuclease and putative chitinase, co-localized with DT QTLs on the chromosome. The expression patterns of some of the corresponding genes were further analyzed at the mRNA level using real-time RTPCR. The comprehensive results suggested that the differentially displayed proteins might play a role in redox metabolism, photosynthesis, protein degradation, cytoskeleton organization and programmed cell death in the DT mechanism of rice.

並列關鍵字

Drought ； DT QTL ； Mass spectrometry ； Proteomics ； Rice ； Two-dimensional gel electrophoresis