A new natural hybrid from the Hengshan Mountain in Hunan Province, China, was found. Comparisons of morphology, karyotype, and ITS sequences among the hybrid and its parental species show that the hybrid represents F1 progeny from hybridization between Aster ageratoides var. scaberulus and Kalimeris indica. Based on morphological and karyotypical observations of the hybrid, this study suggests that natural hybridization between Aster and Kalimeris could have lead to the shortening of the pappus and a special karyotype (LS-type) and makes the delimiting of Aster and Kalimeris difficult. This study also indicates that karyotype investigations may be particularly useful in resolving some of the taxonomic confusion between Aster and Kalimeris.