蛋白酵素及非傳統性蛋白質原料於肉雞飼糧之應用性

Protease and Nonconventional Protein Stuff Applied in the Broiler Diet

張嗣瑜(Szu-Yu Chang) ； 李滋泰(Tzu-Tai Lee) ； 余碧(Bi Yu)

《明道學術論壇》 6卷1期 (2010/03) Pp. 57-75

https://doi.org/10.6953/MJ.201003.0058

摘要

The purpose of study was used nonconventional protein stuff including sunflower meal (SFM) and canola meal (CM) to replace soybean meal (SBM) in broiler diet, meantime, the experimental diet was formulated into an iso-nitrogenic semi-purified diet with the same protein and exogenous proteases supplement used in the in vivo trial. The protease was added either bromelain or microbe protease. Ninety, fourteen day old and 45, thirty-five day old broilers of Arbor Acer used for the in vivo availability test were randomly divided into 9 groups for a 3×3 factorial experiment. The result showed that the protein and amino acid availability of SFM group was higher than SBM and CM group in the growing and finishing periods (P＜0.05). The plasma total free amino acid concentration taken in the first hour after a meal had same trend as the availability results (P＜0.05). Compared with the control group, the supplement of microbial protease had a significant effect on improving protein and amino acid availability and total free amino acids in the plasma than bromelain, it supplement showed no improvement on protein and amino acid availability. Accordingly, availability of protein, amino acids and plasma free amino acids showed the possibility in SFM and FM to replace the traditional SBM with the limited amino acid and dietary fiber to individual considerations; it may be appropriate or in part replaced in order to reduce feed costs. Therefore, nonconventional protein stuffs applied with an appropriate exogenous protease and confirmed by biological test will be improved on feeding effect.

關鍵字

非傳統性蛋白質原料 ； 血漿游離胺基酸 ； 肉雞

並列摘要

The purpose of study was used nonconventional protein stuff including sunflower meal (SFM) and canola meal (CM) to replace soybean meal (SBM) in broiler diet, meantime, the experimental diet was formulated into an iso-nitrogenic semi-purified diet with the same protein and exogenous proteases supplement used in the in vivo trial. The protease was added either bromelain or microbe protease. Ninety, fourteen day old and 45, thirty-five day old broilers of Arbor Acer used for the in vivo availability test were randomly divided into 9 groups for a 3×3 factorial experiment. The result showed that the protein and amino acid availability of SFM group was higher than SBM and CM group in the growing and finishing periods (P＜0.05). The plasma total free amino acid concentration taken in the first hour after a meal had same trend as the availability results (P＜0.05). Compared with the control group, the supplement of microbial protease had a significant effect on improving protein and amino acid availability and total free amino acids in the plasma than bromelain, it supplement showed no improvement on protein and amino acid availability. Accordingly, availability of protein, amino acids and plasma free amino acids showed the possibility in SFM and FM to replace the traditional SBM with the limited amino acid and dietary fiber to individual considerations; it may be appropriate or in part replaced in order to reduce feed costs. Therefore, nonconventional protein stuffs applied with an appropriate exogenous protease and confirmed by biological test will be improved on feeding effect.

並列關鍵字

Nonconventional protein stuff ； plasma free amino acids ； broiler