Fatigue life of asphalt mixes in laboratory tests is usually determined by applying a haversine load with a specific frequency (usually 10Hz). However, the pavement structure, loading and environmental conditions affect the shape and duration of horizontal stress and strain pulse at the bottom of asphalt layer. In this study, the effect of most important factors on the duration of longitudinal and transverse response pulses has been explored and some equations with good accuracy were proposed for determining the duration of tensile stress and strain pulse at the bottom of asphalt layer in both longitudinal and transverse direction. To this end, total of 112 analyses were conducted using 3D-Move dynamic analysis program and in each case, longitudinal and transverse response pulses were determined. With respect to this fact that the haversine function usually used in laboratory fatigue tests, this wave shape was fitted to the computed response pulses and duration of stress and strain pulse were determined. Proposed equations can be used for determining the frequency of loading in HMA fatigue tests under both controlled stress and controlled strain modes.