Effects of Ethyl Acetate Portion of Indigofera Pulchra Leaves Extract on blood Glucose Levels of alloxan-induced Diabetic and Normoglycemic Wistar Rats

Y. Tanko ； M. M. Abdulaziz ； A. B. Adelaiye ； M. Y. Fatihu ； K. Y. Musa

《Asian Journal of Medical Sciences》 1卷2期 (2009/09) Pp. 10-14

並列摘要

The ethyl acetate portion of Indigofera pulchra leaves extract (papilionaceae) was assessed for blood glucose levels of alloxan induced diabetic and normoglycemic Wistar rats. Different doses of the extract were (50, 100 and 200 mg/kg) administered after 18 h fast and there blood glucose levels were measured at 0, 2, 4, 8 and 24 h after treatment. Lowest dose (50 mg/kg) administered significant decrease (p＜0.05) in the blood glucose levels of alloxan-induced diabetic and normoglycemic Wistar rats after 24 hours of treatment. The doses of 100 and 200 mg kg-1 did not show any significantly decrease the blood glucose levels of alloxan induced diabetic Wistar rats. The hypoglycemic and antihyperglycemic potentials of the ethyl acetate portion of the extract was comparable to that of biphasic isophane insulin (6. i. u/kg). In relation to the normoglycemic groups there was a significant decrease in the blood glucose level (p＜0.05) with the doses of 50 and 100 mg/kg after treatments, while there was no any significant change with the highest dose of 200 mg/kg after treatments. The preliminary phytochemical screening of the extract revealed the presences of alkaloids tannins and flavonoids. The lethal dose of the extract (LD50) in rats was calculated to be 775 mg/kg bodyweight. Concluded that the ethyl acetate portion of hydromethanolic leaves extract of Indigofera pulchra possess anti-diabetic effects in alloxan- induced in diabetic and normoglycemic rats.

並列關鍵字

Indigofera pulchra ； ethyl acetate hypoglycemic activity ； normoglycemic ； alloxan ； Diabetes mellitus