Biochemical Characterization of Rice Somaclones Resistant to Blast

The aim of the present study was to produce rice somaclones resistant to P. oryza that could be useful to improve rice plants against blast disease and use them in the future breeding programs. In addition, to detect biochemical changes among somaclones and their original cultivars. Mature embryo-derived calli of the three susceptible rice varieties, Sakha 101, Sakha 104 and Riho were stressed with different concentrations of fungal toxin filtrate. Blast-resistant lines of rice were developed by in vitro selection with toxin filtrate and afterward, their callus induction and their regeneration were tested. The percentage of regenerating calli greatly varied depending on variety and toxin concentration. The reduction of regeneration percentages induced by toxin filtrate was more pronounced in the more sensitive variety, Riho. Protein electrophoresis of regenerated plants which were tested for blast resistance in the R2 generation in the greenhouse, revealed six additional protein bands as compared to their original susceptible cultivars. Esterase patterns showed new enzyme bands in the resistant R2 plants, Alcohol dehydrogenase patterns displayed two distinct bands at two activity zones; these patterns possibly occurred due to a genetic activation mechanism which affected blast gene(s) expression. The blast resistant lines should be tested under field conditions before using them in rice blast breeding programs.

並列關鍵字

Fungal toxin filtrate ； in vitro selection ； Oryza sativa ； Pyricularia oryza ； somaclonal variation

國際替代計量

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