Nodulation Efficiency in Term of Nitrogenase Activity of Rhizobium Mutants and their Wild Type

This study aimed to select suitable strains that can be used as inoculants to enhance legume production and simultaneously reduce the use of inorganic fertilizers. It benefited growth of V. radiata and it was more easily cultured on solid and liquid media than any of the other strains tested including R0132 (1112)::Tn5, R0132 (0097)::Tn5 and R0132 (1106)::Tn5. Mutant R0132 (1106)::Tn5 exhibited superior growth promoting ability under extreme environmental condition; therefore it has potential to be used in India. A slow growing nitrogen-fixing strain of Vigna radiata (mung bean), Rhizobium fredii which expressed nitrogenase activity in a synthetic medium was isolated from its native population. Mutants with decreased and increased nitrogenase activity were derived from this strain by Tn5 mutagenesis. These mutants were tested for in vivo symbiotic activity. The efficiency of mutants R0132 (1106)::Tn5 with increased nitrogenase activity in the culture medium was higher than the parent strain (R0132); This suggests that the plant is perhaps a limiting factor in the full expression of rhizobial nitrogenase in the nodules. Currently involved in researen aimed at improving symbiotic nitrogen fixation (SNF) and root nodule sustainability in Vigna radiate.L.

並列關鍵字

Nitrogenase activity ； symbiotic activity ； inoculation ； mutant strains ； nodulation and nitrogen fixation

國際替代計量

全文下載

主題瀏覽