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Genetic Diversity of Hexaploid Wheat Based on Polymorphism in Quality Characteristics

並列摘要


High Molecular Weight Glutenin Subunits (HMW-GS) were used as markers to assess the genetic diversity among 52 local wheat genotypes and their yield producing capacity with the object of exploiting diversity in the commercial varieties and landraces grown in different regions of Pakistan. HMW-GS profiling of wheat genotypes was done through SDS-PAGE; polymorphism was revealed at HMW-GS encoding loci; Glu-A1, Glu-B1 and Glu-D1; alleles 3, 6 and 4 were identified, respectively. The most common composition of HMW-GS in the population was 2*, 17+18, and 2+12. ANOVA revealed significant variation among the varieties for yield parameters and also there is correlation found between these parameters. The average intrapopulation heterozygosity for the three Glu-1 loci was high in Punjab (60.36%) compared to the other populations that is Sindh (48.88%), Baluchistan (33.33%), Azad Jammu Kashmir (30.36%) and Khyber Pakhtunkhwa (55.98%). Among populations Punjab had maximum genetic similarity of 93.04% with Sindh thus a small genetic distance of 7.21% which showed that the two populations are more identical. Genetic distance was large between the population of Azad Jammu Kashmir and Khyber Pakhtunkhwa. Though the bread-making quality of wheat is good but the heterogeneity is low among the wheat varieties of Pakistan showing a need for improving the genetic pool of the local genotypes for the future breeding programs.

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