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Research on Extraction and Characterization of Cellulase from Commercial Enzyme Preparation
摘要
The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55°C, optimal pH of 4.8, the ions of Co^(2+), Mn^(2+) in the buffer lowered the activity of CMCase, but the Cu^(2+) in low concentration activated the CMCase. The ions of Co^(2+), Mn^(2+) and Al^(3+) in the buffer lowered the activity of FPA, but the Cu^(2+) and K^+ in low concentration activated the FPA; Km and V_(max) of the enzyme were 2.22×10^(-3)g/mL and 1.11×10^4 U/h using Lineweaver-Burk graphic method, respectively.
延伸閱讀
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