- 期刊
- OpenAccess
Application of TLC in the Screening of Acarbose-producing Actinomycetes
並列摘要
Acarbose is widely used in medicine, such as the treatment of diabetes and obesity. A simple Thin Layer Chromatography (TLC)-scanning technique was developed for the rapid and accurate analysis of acarbose in a large number of fermentation broths of actinomycetes to screen for acarbose producer. The linearity of the acarbose in this way was good within the range from 2 to 10 μg (r^2 = 0.9997). This technique didn't need expensive instrument and complex procedure for the detection of acarbose in the fermentation broths. An actinomycete with acarbose yield of 1.83 mg/mL was obtained. The results demonstrated that TLC-scanning was a cheap and simple technique for the accurate screening of acarbose-producing actinomycetes.
延伸閱讀
- 吳榮宗、黃銘郁、林建琛（2008）。Study on the Application of Acidic Ionic Liquid for the Synthesis of Adamantane。石油季刊，44(2)，17-28。https://doi.org/10.30128/JP.200806.0002
- Vimal, V., Rajan, B. M., & Kannabiran, K. (2009). Antimicrobial Activity of Marine Actinomycete, Nocardiopsis sp. VITSVK 5 (FJ973467). Asian Journal of Medical Sciences, 1(2), 57-63. https://www.airitilibrary.com/Article/Detail?DocID=20408773-200909-201009060062-201009060062-57-63
- 許碩辰（2006）。Cytotoxic screening of 2,6-Disubstituted Amidoanthraquinones and Anticancer Molecular Mechanisms Studies.〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-2906200601235100
- 李勃毅（2018）。Studies on the toxicity of inorganic arsenic to Sarcodia suieae〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/THE.NPUST.AQ.002.2018.D08
- Gong, Y. A. (2007). Production of Poly(hydroxybutyrate-co-hydroxyhexanoae) using Aeromonas sp. KC014 [master's thesis, Yuan Ze University]. Airiti Library. https://doi.org/10.6838/YZU.2007.00318