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Establishment of a Rapid Polymerase Chain Reaction Method for the Detection of Staphylococcus aureus in Food

並列摘要


With the improvement of living standards and frequently occurrence of food safety incidents, People pay more and more attention to food safety. Staphylococcus aureus is one of pathogens which could cause food spoilage. In this study, specifically primers has been designed and a rapid method has been established to detect S. aureus in food to shorten time of detection, increase the sensitivity of method and provide a technical help. PCR method has higher specificity, sensitivity, quickness and convenience, which may provide a new approach to detect SA in food quickly. Staphylococcus aureus was detected by PCR samples from food poisoning, in the case of the exclusion of other pathogens that can prompt this food poisoning is caused by Staphylococcus aureus. S. aureus enterotoxin is the main cause of food poisoning, which can be identified on its type enterotoxin produced under conditions permitting.

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