應用聚合酶鏈鎖反應 (PCR) 檢測金黃色葡萄球菌菌株的毒素基因

Application of Polymerase Chain Reaction (PCR) to Detect Toxin Genes in Staphylococcus aureus Isolates

蘇可幼(Ratih Handayani Soekotjo)

指導教授 ： 廖明輝

國立屏東科技大學/農學院/獸醫學系所/碩士(2012年)

https://doi.org/10.6346/NPUST.2012.00250

摘要

應用聚合酶鏈鎖反應 (PCR) 檢測金黃色葡萄球菌菌株的毒素基因

關鍵字

聚合酶鏈鎖反應 ； 金黃色葡萄球菌 ； 毒素基因

並列摘要

In Staphylococcus aureus is a common pathogen that colonizes and cause foodborne disease in a variety of hosts. Staphylococcus aureus is one of the common agents of food poisoning. It may contain one or more genes that encodes a variety of toxin, including the Staphylococcal enterotoxins. The most common types and the most important ones with respect to foodborne illness belong to the family of heat stable Staphylococcal enterotoxins (SEs) five serologically distinct enterotoxins sea, seb, sec, sed and see. It may be important to identify the toxin types for Staphylococcal infection from the epidemiological viewpoint and to determine if there is Staphylococcus aureus present in a suspected vehicle of a foodborne disease outbreak. This study aimed to detect enterotoxin genes and leukocidin toxin gene in Staphylococcus aureus isolates from patients at Pingtung Regional Teaching Hospital, Pingtung City, Taiwan from January 2009 to December 2009.
A PCR assay for detection of genes for sea, seb, sec, sed, see and leukocidin toxin gene were developed. A total of 220 different Staphylococcus aureus isolates encompassing 34 blood isolates, 72 isolates taken from respiratory tract, 82 isolates taken from pus, 12 isolates taken from urine and 20 isolates taken from others were systematically searched by PCR for the staphylococcal enterotoxin (SE) genes sea, seb, sec, sed, see and leukocidin toxin gene. The sizes of the amplified PCR product were 102 bp, 164 bp, 451 bp, 278 bp, 209 bp and 269 bp for sea, seb, sec, sed, see and leukocidin toxin gene, respectively.
Of the 220 strains studied, the gene coding for leukocidin toxin genes (94 isolates, 42%) and seb (66 isolates, 30%) were frequently, followed by sec (47 isolates, 21%), sea (39 isolates, 17%), see (30 isolates, 13%) and sed (0%). For 220 human isolates of Staphylococcus aureus, respiratory tract isolates was the most gene coding for sea, sec, see and leukocidin toxin gene, pus isolates was the most gene coding for seb and no one have gene coding for sed. In the present study, PCR is a feasible tool and can rapidly and specifically detect genes for sea, seb, sec, sed, see and leukocidin toxin gene which associated with human disease.

並列關鍵字

polymerase chain reaction (PCR) ； Staphylococcus aureus ； toxin genes

參考文獻

Akineden O. Toxin genes and other characteristics of Staphylococcus aureus. J Bacteriol 8: 959–964, 2008.

Akineden O. Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis. J Bacteriol 8: 959–964, 2001.

Argudin MA. Review articles:food poisoning and Staphylococcus aureus enterotoxins. J Toxins 2: 1751–1773, 2010.

Arvidson S, Tegmark K. Regulation of virulence determinants in Staphylococcus aureus. Int J Med Microbiol 291:159–170, 2001.

Balaban N, Rasooly A. Staphylococcal enterotoxins. Int J Food Microbiol 61: 1–10, 2000.