The study conducted to obtain Lactobacillus species from bovine colostrum as probiotic candidate that have resistant character towards acidity and bile salt condition. Research done experimentally with Factorial Completely Randomized Design Pattern. The results were analyzed using Analysis of Variance (ANOVA) followed by Duncan's Multiple Range Test (DMRT). Results showed that L. paracasei and L. curvatus have tolerance of pH 2 with colony population by 0.07×10^2 CFU/mL. L. paracasei and L. curvatus also have tolerance as well towards 0.3% of bile salt concentration with colony population by 4.05×10^(10) CFU/mL and 0.5% of bile salt concentration with colony population by 4.02×10^(10) CFU/ml. L. paracasei and L. curvatus have character as probiotic because grew well at high acidity and bile salts condition.