Protective Effect of Penta-acetyl Geniposide on Acute Liver Injury Induced By D-galactosamine in Mice

Hong Zhang ； Tianlu Shi ； Jing Wang ； Rong Li ； Wenjian Tang

《British Journal of Pharmacology and Toxicology》 4卷6期 (2013/12) Pp. 256-261

並列摘要

Penta-acetyl geniposide ((Ac)_5GP), an herbal derivative prepared from Gardenia Fructus geniposide, decreased the DNA damage and hepatocarcinogenesis induced by aflatoxin B1 (AFB_1). The present study was carried out to further evaluate the protective effect of (Ac)_5GP on liver injury induced by D-galactosamine (DGalN). D-GalN (400 mg/kg) was given to mice by intraperitoneal injection, while (Ac)_5GP were orally administered by gastric gavage. Spectrophotometrical method was used to measure activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) in serum and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) in hepatic tissue. Hepatic histological structure was observed under light microscopy. The features of acute liver injury were observed in D-GalN-treated mice. (Ac)_5GP, similar to silymarin, decreased the elevated serum levels of ALT, AST and MDA and increased the reduced activities of SOD and GSH-Px induced by D-GalN. (Ac)_5GP was also showed to be more effective than GP in reversing these abnormal changes and the levels of (Ac)_5GP on MDA and SOD showed a dose-effect relationship. These biochemical observations were also supplemented by histopathological observations of the liver sections. It was concluded from the results that (Ac)_5GP can produce protective effect on acute liver injury induced by D-GalN via an antioxidative mechanism. Therefore, (Ac)_5GP may be developed as an efficient hepato-protective agent.

並列關鍵字

Antioxidative effect ； D-galactosamine ； gardenia ； liver injury ； penta-acetyl geniposide