應用ISSR分子標誌研究台灣檫樹之族群遺傳變異

Genetic Diversity in Sassafras randaiense (Hay.) Rehder (Lauraceae) Based on ISSR Fingerprinting

何坤益(Kuen-Yih Ho) ； 蔡忠誠(Chung-Cheng Tsai) ； 呂福原(Fu-Yuan Lue)

《台灣生物多樣性研究》 12卷4期 (2010/10) Pp. 407-417

https://doi.org/10.7064/TJB.201010.0407

摘要

Sassafras randaiense (Hay.) Rehder (Lauraceae) is an endemic species of Taiwan. We collected 137 individuals from 7 natural populations and examined ISSR fingerprintings of the DNA sequences. PCR amplification with 8 ISSR primers yielded 39 DNA fragments, of which 38 were polymorphic (97.44%). AMOVA revealed that variance component among the populations was 16.47% (p＜0.0001), while within population was 83.53% (p＜0.0001). Mantel test exhibited low correlation (r=0.2830, p=0.1542) between the genetic variation and the spatial distribution. Popgene analysis revealed high genetic diversity (H=0.3438) of the species and also high genetic differentiation among the populations (Gst=0.2788). Cluster analysis and the principal coordinate analysis (PCOA) revealed no significant correlation (r=0.6635 p=0.9995) between the genetic differentiation and the spatial distribution. The conservation strategy for the species is to maintain in situ biodiversity and further to raise its gene diversity by hybridization among the populations in ex situ conservation.

關鍵字

台灣檫樹 ； 簡單序列重複區間 ； 基因歧異度 ； 遺傳分化

並列摘要

Sassafras randaiense (Hay.) Rehder (Lauraceae) is an endemic species of Taiwan. We collected 137 individuals from 7 natural populations and examined ISSR fingerprintings of the DNA sequences. PCR amplification with 8 ISSR primers yielded 39 DNA fragments, of which 38 were polymorphic (97.44%). AMOVA revealed that variance component among the populations was 16.47% (p＜0.0001), while within population was 83.53% (p＜0.0001). Mantel test exhibited low correlation (r=0.2830, p=0.1542) between the genetic variation and the spatial distribution. Popgene analysis revealed high genetic diversity (H=0.3438) of the species and also high genetic differentiation among the populations (Gst=0.2788). Cluster analysis and the principal coordinate analysis (PCOA) revealed no significant correlation (r=0.6635 p=0.9995) between the genetic differentiation and the spatial distribution. The conservation strategy for the species is to maintain in situ biodiversity and further to raise its gene diversity by hybridization among the populations in ex situ conservation.

並列關鍵字

Sassafras randaiense ； ISSR ； genetic diversity ； genetic differentiation