The main purposes of this study were to develop a theory-based measure of self-objectification, and explore potential association between female college students' self-objectification and mental health. A sample of 420 female college students from Kaohsiung city completed self-report measures of self-objectification and mental health. The obtained data were analyzed by confirmatory factor analysis and structural equation modeling. Statistical analysis yielded the following results. First, second-order one factor and first-order three factor model (second-order measurement model) of self-objectification performed adequate goodness of fit. Second, cross-validation of self-objectification second-order measurement model is confirmed. Third, there was significantly negative correlation between self-objectification and mental health of female college students. Based on these results, implication for regulation of female college students' self-objectification and future research was discussed.