- 期刊
應用ITS序列鑑定防己藥材及其製劑
Identificaiton of ＂Stephania tetrandra＂ and Its Preparations by PCR-amplified Its Region Sequencing Method
摘要
防己藥材基原為防己(＂Stephania tetrandra＂ S. Moore)之乾燥塊根，惟「木防己湯」則需使用木防己(＂Cocculus orbiculatus＂ (L.) DC.)，另有易混淆藥材如青風藤(＂Sinomenium acutum＂ Rehder et Wilson)、廣防己(＂Aristolochia fangchi＂ Y.C Wu ex L.D.Chou et S. M. Hwang)及瘤枝微花藤＂Iodes seguini＂ (H. Lév.) Rehd⋯等。利用比對ITS序列鑑定共20件標示防己或粉防己之藥材，其中2件藥材檢體未檢出防己藥材序列。依此段序列設計防己專一性引子對，可得約300鹼基對之產物，並以Nested PCR鑑定共19件單方或複方防己製劑，亦有2件未檢出防己藥材序列。
並列摘要
Because of the similar appearance or Chinese name, there are several medicinal materials easily confused with stephaniae tetrandrae radix (fangji, ＂Stephania tetrandra＂ S. Moore), including ＂Cocculus orbiculatus＂ (L.) DC., ＂Sinomenium acutum＂ Rehder et Wilson., ＂Aristolochia fangchi＂ Y.C Wu ex L.D. Chou et S. M. Hwang, ＂Iodes seguini＂ (H. Lév.) Rehd and so on. By sequencing amplified ITS sequence, of 20 raw material samples, 18 were identified as ＂S. tetrandra＂, one ＂S. acutum＂ and one ＂I. seguini＂. Based on ITS sequences of fangji and those confusing materials, primers specific for ＂S. tetrandra＂ were designed and have the PCR product of nearly 300 bp. The result of Nested PCR with ＂S. tetrandra＂ -specific primers showed 17 out of 19 sample preparations contained ＂S. tetrandra＂.
延伸閱讀
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