以UHPLC-QTOF/MS建立肉蓯蓉類藥材指紋圖譜及市售藥材鑑別之應用

Chemical Fingerprint Analysis for Differentiation of Cistanche Species by UHPLC-QTOF/MS

王博誠(BO-CHENG WANG) ； 林雅姿(YA-TZE LIN) ； 高靖傑(CHING-CHIEH KAO) ； 溫彩玉(CAI-YU WEN) ； 蔡佳芬(CHIA-FEN TSAI) ； 林美智(MEI-CHIH LIN) ； 曾素香(SU-HSIANG TSENG) ； 王德原(DER-YUAN WANG)

《食品藥物研究年報》 10期 (2019/12) Pp. 138-146

摘要

肉蓯蓉具良好滋補功能，常用於輔助治療腎虛或性功能障礙，但嚴重的混誤用情形已經成為肉蓯蓉品質管理的問題之一，甚至影響到病人用藥安全。近年來，肉蓯蓉因過度採集導致野生肉蓯蓉數量銳減，造成市面上混誤用率的增加。根據臺灣中藥典第三版，肉蓯蓉正品應為植物肉蓯蓉Cistanche deserticola Y. C. Ma或管花肉蓯蓉Cistanche tubulosa（Schrenk）Wight之帶鱗葉的乾燥肉質莖。本研究利用超高效液相層析－四極桿飛行式質譜儀（UHPLC-QTOF/MS）進行肉蓯蓉類藥材化學成分之指紋圖譜分析，於肉蓯蓉與管花肉蓯蓉藥材中找出共19種指標成分可供比對，並經主成分分析（principal components analysis, PCA）後可發現不同基原之藥材呈現分群區，可區分出肉蓯蓉、管花肉蓯蓉與其他混誤用藥材。本研究結果能應用於日後肉蓯蓉類藥材品質管制檢驗之依據。

關鍵字

肉蓯蓉 ； 化學指紋圖譜 ； 超高效液相層析四極桿／飛行式質譜儀

並列摘要

Cistanches Herba is a common traditional Chinese medicine with superior tonic activity, and used for the treatment of kidney deficiency and sexual dysfunction. However, the counterfeiting of Cistanches Herba is so rampant in the market and causes problems in the quality control and patient safety. In the recent years, the quantity of Cistanche had been decreasing due to over-harvesting and resulting in severe counterfeiting. According to Taiwan Herbal Pharmacopeia (3^(rd) edition), authentic Cistanches Herba was the dried stem, with scales, of Cistanche deserticola Y. C. Ma or Cistanche tubulosa (Schrenk) Wight. This study aimed at the development for chemical fingerprints of Cistanches Herba by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/ MS). Nineteen characteristic compounds were identified. The data was further analyzed by principal component analysis (PCA) to transform a set of values of linearly uncorrelated variables which was able to differentiate Cistanche deserticola Y. C. Ma and Cistanche tubulosa (Schrenk) Wight from other misused species. This study demonstrated fingerprinting technique can be used for quality control and then enhancing the safety of Cistanches Herba products.

並列關鍵字

Cistanches Herba ； chemical fingerprint ； ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry