以UHPLC-QTOF/MS建立茵陳類藥材指紋圖譜及其市售藥材應用

Identification of Artemisiae Scopariae Herba Using Chemical Fingerprint by UHPLC-Q-TOF/MS

王博誠(BO-CHENG WANG) ； 林雅姿(YA-TZE LIN) ； 溫彩玉(CAI-YU WEN) ； 盧芬鈴(FEN-LING LU) ； 蔡佳芬(CHIA-FEN TSAI) ； 林美智(MEI-CHIH LIN) ； 王德原(DE-YUAN WANG) ； 陳惠芳(HWEI-FANG CHENG)

《食品藥物研究年報》 9期 (2018/12) Pp. 196-205

摘要

臺灣中藥典記載藥材茵陳為菊科植物濱蒿(Artemisia scoparia Waldst. Et Kit.)或茵陳蒿(Artemisia capillaris Thunb.)之乾燥地上部分。茵陳藥材與青蒿(Artemisia annua L.)其基原與外觀型態相近，而牛至(Origanum vulgaris L.)別名為北茵陳、土茵陳等，導致市面上常出現同名異物的混淆情況，為避免藥材使用錯誤而影響療效，本研究蒐集市售茵陳蒿及其常見誤用品總計4品項36件藥材，以超高效液相層析串聯四極桿／飛行式質譜(Ultra-high Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry, UHPLC-Q-TOF/MS)建立指紋圖譜鑑定方法，並針對指紋成分以主成分分析之歸屬結果進行比較。研究結果顯示，市售茵陳蒿藥材經指紋圖譜比對之混誤用率為87.5% (n=8)，且鑑別結果與主成分分析之歸屬結果一致，可作為日後品質管制檢驗之依據。

關鍵字

茵陳 ； 指紋圖譜 ； 超高效液相層析串聯四極桿／飛行式質譜

並列摘要

Artemisiae scopariae herba is the dried aerial part of Artemisia scoparia Waldst. or Artemisia capillaris Thunb. However, it is often confused with other Artemisia species because of their similar outward appearances. It is also confused with Origanum vulgare L. due to similar common names. To prevent misuse of the herb, in this study, thirty-six samples of artemisiae scopariae herba collected from market were identified using chemical fingerprint by UHPLC-Q-TOF/MS combined with principal component analysis (PCA). The results showed the misuse rate of Artemisia scoparia Waldst. was 87.5% (n=8). This result corresponded with PCA, which showed that chemical fingerprint by UHPLC-Q-TOF/ MS could be used as reference for quality control in the future.

並列關鍵字

artemisiae scopariae herba ； fingerprint ； UHPLC-Q-TOF/MS