利用液相層析串聯質譜法分析化粧品中之雌激素

Determination of Estrogens in Cosmetics by Liquid Chromatography-Tandem Mass Spectrometry

陳信豪(Shin-Hao Chen) ； 黃守潔(Shou-Chieh Huang) ； 陳玉盆(Yu-Pen Chen) ； 闕麗卿(Lih-Ching Chiueh) ； 施養志(Daniel Yang-Chih Shih)

《食品藥物研究年報》 5期 (2014/12) Pp. 299-306

摘要

本研究係採用液相層析串聯質譜法(liquid chromatography-tandem mass spectrometry, LC/MS/MS)，以正／負離子電灑法(positive/negative mode)之多重反應偵測(multiple reaction monitoring,MRM)模式同時檢測化粧品中Estriol、Estradiol、Estrone、Ethinyl estradiol、Diethylstilbestrol、Pregnanediol、Progesterone及Estradiol benzoate 8種雌激素(Estrogens)成分之檢測方法。使用之層析管柱為Waters UPLC BEH C18（1.7 μm,內徑2.1 mm×100 mm）；移動相為去離子水和乙腈進行梯度流洗；流速為0.3 mL/min；本研究方法中針對8種雌激素成分檢測，可在10分內鐘完成分析，標準曲線之線性範圍為0.005-1.0 μg/mL，標準曲線線性回歸係數r^2皆為0.9953以上。添加不同濃度之標準品於空白乳液中進行三重複試驗，回收率介於85.2-115.0%，相對標準偏差均小於8.8%。

關鍵字

液相層析串聯質譜法 ； 雌激素 ； 化粧品

並列摘要

A method for the analysis of 8 estrogens in cosmetics by liquid chromatography-tandem mass spectrometry (LC/MS/MS) with electro spray ionization was developed. Data acquisition under MS/MS for each estrogen was achieved by applying multiple reaction monitoring (MRM). Estriol、estradiol、 ethinyl estradiol、estrone、diethylstilbestrol、progesterone and estradiol benzoate were extracted from cosmetics with methanol, and analyzed by LC/MS/MS with electrospray ionization.The LC separation was conducted on an Waters ACQUITY BEH C18 (I.D. 2.1 mm × 100 mm, 1.7 μm) column with gradient elution of water and acetonitrile as the mobile phase. The concentration range of standards were 0.005-1.0 μg/mL with an r^2 value greater than 0.9953. The average recoveries of the 8 estrogen spiked in instant creams ranged from 85.2-115.0% and the relative coefficients of variation of all estrogens were below 8.8%.

並列關鍵字

LC/MS/MS ； estrogen ； cosmetics