光照度對硝基藍四氮唑光照反應的影響

The Effect of Illuminance on Light Induced Reduction of Nitro Blue Tetrazolium

鄭建瑋(Jian-Wei Juen) ； 簡宏霖(Hong-Lin Jian) ； 梁致遠(Ji-Yuan Liang)

《MC-Transaction on Biotechnology》 2卷1期 (2010/03) Pp. 11-21

https://doi.org/10.29690/MCTB.201003.0002

摘要

以光照激發核黃素產生超氧自由基的方式，研究硝基藍四氮唑光照還原法的影響因子。結果表明，光照度、硝基藍四氮唑濃度及緩衝溶液的pH會影響硝基藍四氮唑光照還原反應後的吸光值。相同光照度，隨著硝基藍四氮唑的濃度增加，光照還原反應後吸光值平均速率遞增。在pH 7.8、高濃度硝基藍四氮唑條件，隨著光照度的增加，光照還原反應後吸光值平均增加速率隨之增加；低濃度硝基藍四氮唑，雖提高光照度，但能夠反應的硝基藍四氮唑有限，光照還原反應後吸光值平均速率無法隨光照度增加而增加。在相同光照度，pH 7.8條件下的光照還原反應後吸光值平均增加速率較pH 7.0者為高。

關鍵字

核黃素 ； 硝基藍四氮唑 ； 光照度

並列摘要

Superoxide radicals generated from light-exited riboflavin were used to study the effect of illuminance on the light reaction of nitro blue tetrazolium (NBT). The pH value of buffer, illuminance and the concentrations of NBT were found the major factors correlated with the absorbance of the NBT solution after light induced reduction. At a fixed illuminance, the increasing rate of absorbance of NBT reduction is increased with the NBT concentration. At pH 7.8 and higher NBT concentrations, the increasing rates of absorbance of NBT reduction were raised with the enhanced illuminations. However, at lower concentrations of NBT, the available NBT for reaction was limited, though the illuminance had been enhanced. The increasing rates of absorbance of NBT reduction were not increased with the illuminance.. At a same strength of illuminance, the increasing rates of absorbance of NBT reduction at pH 7.8 were higher than those at pH 7.0.

並列關鍵字

riboflavin ； NBT ； Illuminance