- 學位論文
硝基四氮唑藍及光降解產物之電化學量測研究
Electrochemical measurement of nitro blue tetrazolium and photodegradation products
摘要
本實驗利用電化學探討硝基四氮唑藍(NBT)光化學還原法檢測超氧歧化酶(SOD)系統在石英晶片金電極與網版印刷碳電極上的電化學變化。主要是利用核黃素易受到光照分解產生超氧陰離子，而超氧陰離子會與硝基四氮唑藍和超氧歧化酶反應。 利用交流阻抗法探討LED藍光燈，對NBT產生formazan(甲䐶)的影響，以及使用能量分散光譜儀(EDS)雙重驗證，使用LED藍光燈進行四種不同光強度探討核黃素光解與NBT產生formazan的關聯。結果表明光強度為200Lux時，照光30min會產生最多的formazan，因此選擇200Lux光強度照光30min為最佳條件，實驗設計以改變硝基四氮唑藍濃度對產生formazan的影響作探討。最後階段在200Lux照光30min下，加入超氧歧化酶(SOD)，探討超氧歧化酶清除超氧陰離子的活性情形。
並列摘要
Discussion in this study, the use of cyclic voltammetry nitro blue tetrazolium (NBT) photochemical reduction assay superoxide dismutase (SOD) activity in the gold electrode electrochemical changes and glassy carbon electrode. Mainly using riboflavin susceptible to light decomposition superoxide anion. Use electrochemical impedance spectrum(EIS) with blue LED, and the use of energy dispersive spectroscopy (EDS) to double check. We use of the blue LED explore four different light intensities produce riboflavin photolysis with NBT formazan association. The results show that when the light intensity is 200Lux, illuminated 30min produces the most formazan, so choose 200Lux light intensity illumination 30min for the best conditions, the experimental design that generated formazan impacted by changing the nitro blue tetrazolium concentration. In the final stage of illumination 30min 200Lux under different concentrations of superoxide dismutase (SOD), superoxide dismutase (SOD) investigate the case of superoxide anion scavenging activity.