Riboflavin is sensitive to light. Various compounds may be derived from riboflavin after illumination. This study was working on the effects of luminance and light quality to photo-decomposition of riboflavin and production of reactive oxygen specie, based on the photochemical properties of riboflavin. Due to its reversible redox property, some electrons of riboflavin might be transferred after illumination by ultraviolet or visible lights. The guano purine residue on riboflavin is broken and which results in an altered structure of nucleic acid linkage. Riboflavin photochemistry can be a simple and safe blood cleaning technology.