酸鹼值及氧氣對核黃素光反應的影響

Effects of pH and Oxygen Content on Riboflavin Photolysis

陳良宇(Liang-Yu Chen) ； 陳淑玲(Shu-Ling Chen) ； 簡宏霖(Hong-Lin Jian) ； 梁致遠(Ji-Yuan Liang)

《MC-Transaction on Biotechnology》 8卷1期 (2016/03) Pp. 15-22

摘要

核黃素對光敏感，經藍光照射後，激發核黃素產生超氧自由基，可破壞大腸桿菌質體 DNA，使大腸桿菌失活。本文以緩衝溶液pH、藍光光照強度及氧氣濃度探討核黃素光化學反應產生超氧自由基的影響。結果表明，在同樣藍光強度照射的核黃素，超氧自由基隨著緩衝溶液的pH值增加而提高。在相同的溶液pH，照射的藍光強度增加，核黃素光化學反應產生的超氧自由基增加。低溶氧的緩衝溶液，不影響核黃素光化學反應產生超氧自由基。

關鍵字

核黃素 ； 光照強度 ； 溶氧量

並列摘要

Riboflavin is sensitive to blue light for generating reactive oxygen species and able to inactivate E. coli by damaging nucleic acids with free radicals generated. This study was working on the effects of pH, blue light irradiance intensity and dissolved oxygen on photo-decomposition of riboflavin and production of reactive oxygen species, based on the photochemical property of riboflavin. At a fixed blue light irradiance intensity, the increasing rate of production of superoxide radicals from photodecomposition of riboflavin was increased with the pH of buffer solution. At a fixed pH of buffer solution, the increasing rate of production of superoxide radicals from photo-decomposition of riboflavin was increased with the blue light irradiance intensity. The photo-decomposition of riboflavin and production of reactive oxygen species was not influence by dissolved oxygen in buffer solution.

並列關鍵字

riboflavin ； irradiance intensity ； dissolved oxygen