There are lower immune cells values and recurrent infection, particularly upper respiratory tract infection, after high-load training. However, long-term regular exercise training can increase the concentration of natural killer cells and saliva IgA, IgM, It has long been engaged in regular moderate exercise can promote the body's immune system function, enhance and modulate the immune function, enhance the body's resistance to a variety of pathogens and viruses, reduce morbidity and mortality. Lymphocyte subsets have an important role in the operation of the immune system, therefore, this article will try to link a single exercise and long-term regular exercise on the immune system may be associated with changes in lymphocyte subsets.