Purpose: Using the inertial measurement unit which was fitted on the club to compare with the golfers and beginners' hitting movement in different distances. Methods: Ten golfers and ten beginnerswere required toconduct 3 putting tests, each for 10 times, withthe three putt distances of 90cm, 180cm, and 270cm. The putting movement data were measurement byinertial measurement unit, and the putting kinematic parameters were calculated by the MATLAB software. The data are analyzed by one-way ANOVA test with SPSS 18.0 statistical software. Results: The kinematic parameters in all putt distance, beginners and golfers all reached significant difference (p <.05). When the distance was increased between in two groups makes more significant differences (p <.01). Conclusion: It is a way to measure the putt movement by using inertial measurement unit, and hope we can provides a new method for another research in the future.